The preservation of microbial DNA in archived soils of various genetic types

Autor: Boris Aparin, Elizaveta Pershina, Ekaterina Ivanova, Nikolai A. Provorov, Evgeny E. Andronov, Evgenii L. Chirak, Ilia Korvigo, Nikolay S. Romaschenko
Rok vydání: 2016
Předmět:
0301 basic medicine
Microbial DNA
Biodiversity
lcsh:Medicine
Biochemistry
Soil
Agricultural Soil Science
lcsh:Science
Paleopedology
Soil Microbiology
Multidisciplinary
Ecology
Microbial Genetics
Soil classification
Agriculture
04 agricultural and veterinary sciences
Genomics
Actinobacteria
Nucleic acids
Agricultural soil science
Ribosomal RNA
Medical Microbiology
Soil microbiology
Research Article
DNA
Bacterial
Cell biology
Cellular structures and organelles
030106 microbiology
Preservation
Biological
Soil Science
Microbial Genomics
Biology
complex mixtures
Microbiology
03 medical and health sciences
Genetics
Non-coding RNA
Chernozem
Bacteria
lcsh:R
Ecology and Environmental Sciences
Organisms
Biology and Life Sciences
Paleontology
Soil water
040103 agronomy & agriculture
Earth Sciences
0401 agriculture
forestry
and fisheries
RNA
lcsh:Q
Microbiome
Ribosomes
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 3, p e0173901 (2017)
ISSN: 1932-6203
Popis: This study is a comparative analysis of samples of archived (stored for over 70-90 years) and modern soils of two different genetic types-chernozem and sod-podzolic soils. We revealed a reduction in biodiversity of archived soils relative to their modern state. Particularly, long-term storage in the museum exerted a greater impact on the microbiomes of sod-podzolic soils, while chernozem samples better preserved the native community. Thus, the persistence of microbial DNA in soil is largely determined by the physico-chemical characteristics that differ across soil types. Chernozems create better conditions for the long-term DNA preservation than sod-podzolic soils. This results in supposedly higher levels of biodiversity conservation in the microbiomes of chernozem with preservation of major microbial taxa dominant in the modern (control) soil samples, which makes archived chernozems a promising object for paleosoil studies.
Databáze: OpenAIRE
Externí odkaz: