Denervation in Femoral Artery-Ligated Hindlimbs Diminishes Ischemic Recovery Primarily via Impaired Arteriogenesis

Autor: Yuansen Qin, Rui-Ming Liu, Shenming Wang, Ying-huan Cen, Junfeng Liu, Zuojun Hu, Huijin Wang, Yu Zhou
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Fluorescent Antibody Technique
lcsh:Medicine
Hindlimb
Femoral artery
030204 cardiovascular system & hematology
Nervous System
Vascular Medicine
Rats
Sprague-Dawley
0302 clinical medicine
Ischemia
Medicine and Health Sciences
lcsh:Science
Musculoskeletal System
Denervation
Multidisciplinary
Nerves
Muscles
Anatomy
Arteries
Collateral circulation
Peripheral
Femoral Artery
Perfusion
Platelet Endothelial Cell Adhesion Molecule-1
Cardiology
Legs
Female
Research Article
medicine.medical_specialty
Myocytes
Smooth Muscle
Collateral Circulation
Neovascularization
Physiologic
Surgical and Invasive Medical Procedures
03 medical and health sciences
Sciatic Nerves
medicine.artery
Internal medicine
medicine
Animals
Peripheral Nerves
Ligation
Cell Proliferation
business.industry
Limbs (Anatomy)
lcsh:R
Endothelial Cells
Biology and Life Sciences
Recovery of Function
Femoral Arteries
medicine.disease
Capillaries
030104 developmental biology
Cardiovascular Anatomy
Blood Vessels
lcsh:Q
Arteriogenesis
business
Zdroj: PLoS ONE, Vol 11, Iss 5, p e0154941 (2016)
PLoS ONE
ISSN: 1932-6203
Popis: Aims Multiple factors regulate arteriogenesis. Peripheral nerves play a crucial role in vascular remodeling, but the function of peripheral nerves during arteriogenesis is obscure. Our study investigated the contribution of denervation to arteriogenesis during post-ischemic recovery from hindlimb femoral artery ligation. Methods and Results Sprague-Dawley rats were randomly allocated into four groups of normal control (NC), hindlimb ischemia (HI), hindlimb ischemia with denervation (HID) and hindlimb simple denervation (HD). Hindlimb ischemic recovery was assessed by clinical assessment and tibialis anterior muscle remodeling on day 28 post-surgery. Blood flow was determined by laser Doppler imaging on day 0, 3, 7, 14 and 28 post-surgery. Collateral number of hindlimb was observed by angiography and gracilis muscles were tested by immunostaining on day 7 and 28 post-surgery. Angiogenesis was accessed by counting CD31 positive capillaries in tibialis anterior muscles on day 28 post-surgery. Group HID showed impaired ischemic recovery compared with the other 3 groups and impaired blood flow recovery compared with group HI on day 28 post-surgery. The collateral number and capillary density of group HID were lower than group HI. The collateral diameter of both group HID and group HI significantly increased compared with group NC. However, the lumen diameter was much narrower and the vessel wall was much thicker in group HID than group HI. We also demonstrated that the thickened neointima of collaterals in group HID comprised of smooth muscle cells and endothelial cells. Conclusions Denervation of the ligated femoral artery in the hindlimb impairs ischemic recovery via impaired perfusion. The possible mechanisms of impaired perfusion are lower collateral number, lower capillary density and most likely narrower lumen, which damage ischemic recovery. This study illustrates the crucial role of peripheral nerves in arteriogenesis using a model combined ischemia with denervation in hindlimb.
Databáze: OpenAIRE
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