Epidemiology of yeast species causing bloodstream infection in Tehran, Iran (2015-2017); superiority of 21-plex PCR over the Vitek 2 system for yeast identification
| Autor: | Ahmad Elmimoghaddam, Alireza Abdollahi, Sassan Rezaei, Teun Boekhout, Amir Arastehfar, Mahsa Doomanlou, Sadegh Khodavaisy, Roshanak Daie Ghazvini, Ayda Maleki, Weihua Pan, Seyed Jamal Hashemi, Neda Alijani, Niloofar Rashidi, Mohammadreza Salehi, Mohammad Kord, Kazem Ahmadikia |
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| Přispěvatelé: | Westerdijk Fungal Biodiversity Institute - Yeast Research, Westerdijk Fungal Biodiversity Institute, Evolutionary and Population Biology (IBED, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male History bloodstream infections Fungemia/diagnosis Iran Candida parapsilosis Candida tropicalis Medical Mycology Yeasts 80 and over Candida albicans Aged 80 and over biology General Medicine Middle Aged 21st Century Corpus albicans DNA/methods Iran/epidemiology Sequence Analysis DNA/methods Vitek 2 DNA Intergenic Female Yeasts/classification Fungemia Sequence Analysis medicine.drug Microbiology (medical) Multiplex Polymerase Chain Reaction/methods Short Communication 030106 microbiology Microbiology History 21st Century 03 medical and health sciences 21-plex PCR Candida krusei medicine candidaemia Humans Aged Intergenic Candida glabrata Sequence Analysis DNA DNA biology.organism_classification Yeast 030104 developmental biology Multiplex Polymerase Chain Reaction Fluconazole |
| Zdroj: | Journal of Medical Microbiology, 69(5), 712-720. Society for General Microbiology Journal of Medical Microbiology |
| ISSN: | 0022-2615 |
| DOI: | 10.1099/jmm.0.001189 |
| Popis: | Introduction. Given the limited number of candidaemia studies in Iran, the profile of yeast species causing bloodstream infections (BSIs), especially in adults, remains limited. Although biochemical assays are widely used in developing countries, they produce erroneous results, especially for rare yeast species.Aim. We aimed to assess the profile of yeast species causing BSIs and to compare the accuracy of the Vitek 2 system and 21-plex PCR.Methodology. Yeast blood isolates were retrospectively collected from patients recruited from two tertiary care training hospitals in Tehran from 2015 to 2017. Relevant clinical data were mined. Identification was performed by automated Vitek 2, 21-plex PCR and sequencing of the internal transcribed spacer region (ITS1-5.8S-ITS2).Results. In total, 137 yeast isolates were recovered from 107 patients. The overall all-cause 30-day mortality rate was 47.7 %. Fluconazole was the most widely used systemic antifungal. Candida albicans (58/137, 42.3 %), Candida glabrata (30/137, 21.9 %), Candida parapsilosis sensu stricto (23/137, 16.8 %), Candida tropicalis (10/137, 7.3 %) and Pichia kudriavzevii (Candida krusei) (4/137, 2.9 %) constituted almost 90 % of the isolates and 10 % of the species detected were rare yeast species (12/137; 8.7 %). The 21-plex PCR method correctly identified 97.1 % of the isolates, a higher percentage than the Vitek 2 showed (87.6 %).Conclusion. C. albicans was the main cause of yeast-derived fungaemia in this study. Future prospective studies are warranted to closely monitor the epidemiological landscape of yeast species causing BSIs in Iran. The superiority of 21-plex PCR over automated Vitek 2 indicates its potential clinical utility as an alternative identification tool use in developing countries. |
| Databáze: | OpenAIRE |
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