Increased chondrocyte death after steroid and local anesthetic combination
| Autor: | Tamás Czömpöly, Tamás Bárdos, Boglárka Farkas, Tamás Illés, Krisztian Kvell |
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| Rok vydání: | 2009 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Time Factors medicine.drug_class Cell Survival Prednisolone Apoptosis Osteoarthritis Pharmacology Betamethasone Chondrocyte Necrosis Chondrocytes Internal medicine medicine In Situ Nick-End Labeling Humans Orthopedics and Sports Medicine Ropivacaine Anesthetics Local Glucocorticoids Hyaline Cells Cultured Local anesthetic business.industry Regeneration (biology) Lidocaine Drug Synergism General Medicine musculoskeletal system medicine.disease Flow Cytometry Amides Bupivacaine medicine.anatomical_structure Endocrinology Basic Research Surgery business hormones hormone substitutes and hormone antagonists Glucocorticoid medicine.drug |
| Zdroj: | Clinical orthopaedics and related research. 468(11) |
| ISSN: | 1528-1132 |
| Popis: | Hyaline articular cartilage has limited repair and regeneration capacity. Intraarticular administration of glucocorticoid and local anesthetic injections play an important role in the therapy of osteoarthritis. Glucocorticoids and anesthetics reportedly enhance apoptosis in chondrocytes, but effects of the combined use of glucocorticoids and local anesthetics are unknown.We asked whether glucocorticoid and local anesthetic agents combined had any synergistic effects on chondrocyte apoptosis.Cell viability and apoptosis/necrosis assessment of human articular chondrocytes were performed in vitro (chondrocyte cell cultures) and ex vivo (osteochondral specimens) using flow cytometry and TUNEL analysis, respectively.Glucocorticoids and local anesthetics induce apoptosis in chondrocytes at various rates. When used in combination, the percentage of dead chondrocytes was increased in in vitro chondrocyte cell cultures and osteochondral ex vivo specimens.We observed a time-dependent decrease in chondrocyte viability after concurrent steroid and local anesthetic exposure.The combination of glucocorticoids and local anesthetics has an adverse effect on articular chondrocytes, and it raises a question regarding whether concomitant administration should be used in treating osteoarthritis. |
| Databáze: | OpenAIRE |
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