Enlargement of the WHO international repository for platelet transfusion-relevant bacteria reference strains

Autor:	Dirk de Korte, Charlotte Ingram, B. Lambrecht, K. M. Hanschmann, Birgit S. Gathof, Susanne Süßner, Colin P. McDonald, Z. Mukhtar, K. Hourfar, K. Aplin, Masahiro Satake, Sandra Ramirez-Arcos, Shawn D. Keil, Michael R. Jacobs, R. Yomtovian, Julieta Rojo, T. Niekerk, H. Nagumo, Melanie Stormer, Christian Gabriel, Isabelle Bekeredjian-Ding, Eva Spindler-Raffel, Erhard Seifried, Stephen J. Wagner, Axel Seltsam, S. Marschner, Yuntong Kou, Jan H. Marcelis, Shaheen Sharafat, Richard J. Benjamin
Rok vydání:	2017
Předmět:	Blood Platelets Staphylococcus aureus Blood Safety Bacillus cereus Platelet Transfusion 030204 cardiovascular system & hematology medicine.disease_cause World Health Organization Serratia Microbiology 03 medical and health sciences 0302 clinical medicine Morganella medicine Escherichia coli Staphylococcus epidermidis Humans Biological Specimen Banks biology Streptococcus Hematology General Medicine Enterobacter Reference Standards biology.organism_classification Proteus Klebsiella pneumoniae Enterobacter cloacae 030215 immunology
Zdroj:	Vox sanguinis. 112(8)
ISSN:	1423-0410
Popis:	Background and Objectives Interventions to prevent and detect bacterial contamination of platelet concentrates (PCs) have reduced, but not eliminated the sepsis risk. Standardized bacterial strains are needed to validate detection and pathogen reduction technologies in PCs. Following the establishment of the First International Reference Repository of Platelet Transfusion-Relevant Bacterial Reference Strains (the ‘repository’), the World Health Organization (WHO) Expert Committee on Biological Standardisation (ECBS) endorsed further repository expansion. Materials and Methods Sixteen bacterial strains, including the four repository strains, were distributed from the Paul-Ehrlich-Institut (PEI) to 14 laboratories in 10 countries for enumeration, identification and growth measurement on days 2, 4 and 7 after low spiking levels [10–25 colony-forming units (CFU)/PC bag]. Spore-forming (Bacillus cereusPEI-B-P-07-S, Bacillus thuringiensisPEI-B-P-57-S), Gram-negative (Enterobacter cloacaePEI-B-P-43, Morganella morganiiPEI-B-P-74, PEI-B-P-91, Proteus mirabilisPEI-B-P-55, Pseudomonas fluorescensPEI-B-P-77, Salmonella choleraesuisPEI-B-P-78, Serratia marcescensPEI-B-P-56) and Gram-positive (Staphylococcus aureusPEI-B-P-63, Streptococcus dysgalactiaePEI-B-P-71, Streptococcus bovisPEI-B-P-61) strains were evaluated. Results Bacterial viability was conserved after transport to the participating laboratories with one exception (M. morganiiPEI-B-P-74). All other strains showed moderate-to-excellent growth. Bacillus cereus, B. thuringiensis, E. coli, K. pneumoniae, P. fluorescens, S. marcescens, S. aureus and S. dysgalactiae grew to >106 CFU/ml by day 2. Enterobacter cloacae, P. mirabilis, S. epidermidis, S. bovis and S. pyogenes achieved >106 CFU/ml at day 4. Growth of S. choleraesuis was lower and highly variable. Conclusion The WHO ECBS approved all bacterial strains (except M. morganiiPEI-B-P-74 and S. choleraesuisPEI-B-P-78) for repository enlargement. The strains were stable, suitable for spiking with low CFU numbers, and proliferation was independent of the PC donor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3611ed1c30d44aae4b0cdc3b4f4b3492 https://pubmed.ncbi.nlm.nih.gov/28960367 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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