Structure and hypoglycemic activity of a novel exopolysaccharide of Cordyceps militaris

Autor: Hui-Qing Sun, Ting Li, XiaoFeng Yu, Zhen-Yuan Zhu
Rok vydání: 2021
Předmět:
Blood Glucose
Male
Chromatography
Gas
Proton Magnetic Resonance Spectroscopy
02 engineering and technology
Polysaccharide
Methylation
Biochemistry
Helix structure
Diabetes Mellitus
Experimental
Mice
03 medical and health sciences
Polysaccharides
Structural Biology
In vivo
Spectroscopy
Fourier Transform Infrared
Cordyceps militaris
Animals
Hypoglycemic Agents
Insulin
Glycoside Hydrolase Inhibitors
Food science
Sugar
Molecular Biology
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
biology
Chemistry
Monosaccharides
Periodic Acid
Congo Red
Diabetic mouse
General Medicine
Plasma glucose concentration
Glucose Tolerance Test
Lipid Metabolism
021001 nanoscience & nanotechnology
biology.organism_classification
Molecular Weight
Homogeneous
Cordyceps
0210 nano-technology
Oxidation-Reduction
Zdroj: International Journal of Biological Macromolecules. 166:496-508
ISSN: 0141-8130
Popis: A novel neutral exopolysaccharide (EPS-III) was isolated from culture broth of Cordyceps militaris (C. militaris). The EPS-III was a homogeneous polysaccharide with Mw of 1.56 × 103 kDa. The yield of EPS-III from culture broth was 123.2 ± 3.1 mg/L and the sugar content was 93.32 ± 0.87%. The backbone of EPS-III was mainly consisted of →4)-α-D-Galp-(1→, while →3, 6)-α-D-Manp-(1→, →4)-α-D-Manp-(1→, →3)-β-D-Galp-(1→ and →3)-α-D-Glcp-(1→ were distributed in the backbone or in the branch chains. The EPS-III had helix structure when dissolved in weak alkaline solution. It also had branched and intertwined form on the surface. The inhibition of α-glucosidase significantly increased as the increase of purity of exopolysaccharides. The EPS-III had effective inhibition on the α-glucosidase with dose-effect relationship. Besides, the results of hypoglycemic activity analysis in vivo indicated that EPS-III can alleviate weight loss, reduce plasma glucose concentration, improve glucose tolerance, protect immune organs and repair dyslipidemia to relieve diabetes in STZ-induced diabetic mice. The manuscript first studied the hypoglycemic activity of exopolysaccharide of by C. militaris, proving and promoting the application value of culture broth. The structure characterization of EPS-III laid experimental foundations on the exploration of structure-activity relationship.
Databáze: OpenAIRE
Externí odkaz: