Structure and hypoglycemic activity of a novel exopolysaccharide of Cordyceps militaris
Autor: | Hui-Qing Sun, Ting Li, XiaoFeng Yu, Zhen-Yuan Zhu |
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Rok vydání: | 2021 |
Předmět: |
Blood Glucose
Male Chromatography Gas Proton Magnetic Resonance Spectroscopy 02 engineering and technology Polysaccharide Methylation Biochemistry Helix structure Diabetes Mellitus Experimental Mice 03 medical and health sciences Polysaccharides Structural Biology In vivo Spectroscopy Fourier Transform Infrared Cordyceps militaris Animals Hypoglycemic Agents Insulin Glycoside Hydrolase Inhibitors Food science Sugar Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Chemistry Monosaccharides Periodic Acid Congo Red Diabetic mouse General Medicine Plasma glucose concentration Glucose Tolerance Test Lipid Metabolism 021001 nanoscience & nanotechnology biology.organism_classification Molecular Weight Homogeneous Cordyceps 0210 nano-technology Oxidation-Reduction |
Zdroj: | International Journal of Biological Macromolecules. 166:496-508 |
ISSN: | 0141-8130 |
Popis: | A novel neutral exopolysaccharide (EPS-III) was isolated from culture broth of Cordyceps militaris (C. militaris). The EPS-III was a homogeneous polysaccharide with Mw of 1.56 × 103 kDa. The yield of EPS-III from culture broth was 123.2 ± 3.1 mg/L and the sugar content was 93.32 ± 0.87%. The backbone of EPS-III was mainly consisted of →4)-α-D-Galp-(1→, while →3, 6)-α-D-Manp-(1→, →4)-α-D-Manp-(1→, →3)-β-D-Galp-(1→ and →3)-α-D-Glcp-(1→ were distributed in the backbone or in the branch chains. The EPS-III had helix structure when dissolved in weak alkaline solution. It also had branched and intertwined form on the surface. The inhibition of α-glucosidase significantly increased as the increase of purity of exopolysaccharides. The EPS-III had effective inhibition on the α-glucosidase with dose-effect relationship. Besides, the results of hypoglycemic activity analysis in vivo indicated that EPS-III can alleviate weight loss, reduce plasma glucose concentration, improve glucose tolerance, protect immune organs and repair dyslipidemia to relieve diabetes in STZ-induced diabetic mice. The manuscript first studied the hypoglycemic activity of exopolysaccharide of by C. militaris, proving and promoting the application value of culture broth. The structure characterization of EPS-III laid experimental foundations on the exploration of structure-activity relationship. |
Databáze: | OpenAIRE |
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