A broad-spectrum human lung fibroblast-derived mitogen is a variant of hepatocyte growth factor
| Autor: | William G. Taylor, Toru Miki, Paul Finch, D W Hirschfield, A C Cech, J Wong, Donald P. Bottaro, Andrew K Chan, Wilson H. Burgess, Jeffrey S. Rubin |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
DNA Replication
medicine.medical_treatment Molecular Sequence Data Restriction Mapping Mice Inbred Strains Biology Transfection Cell Line Mice chemistry.chemical_compound Paracrine signalling medicine Animals Humans Growth factor receptor inhibitor Amino Acid Sequence Cloning Molecular Growth Substances Lung Multidisciplinary Hepatocyte Growth Factor Growth factor Genetic Variation Tyrosine phosphorylation DNA Fibroblasts Protein-Tyrosine Kinases Molecular biology Recombinant Proteins Enzyme Activation Kinetics Vascular endothelial growth factor A chemistry biology.protein Hepatocyte growth factor Mitogens Oligonucleotide Probes Tyrosine kinase Platelet-derived growth factor receptor Research Article medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 88:415-419 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.88.2.415 |
| Popis: | A heparin-binding mitogen was isolated from conditioned medium of human embryonic lung fibroblasts. It exhibited broad target-cell specificity whose pattern was distinct from that of any known growth factor. It rapidly stimulated tyrosine phosphorylation of a 145-kDa protein in responsive cells, suggesting that its signaling pathways involved activation of a tyrosine kinase. Purification identified a major polypeptide with an apparent molecular mass of 87 kDa under reducing conditions. Partial amino acid sequence analysis and cDNA cloning revealed that it was a variant of hepatocyte growth factor, a mitogen thought to be specific for hepatic cells and structurally related to plasminogen. Recombinant expression of the cDNA in COS-1 cells established that it encoded the purified growth factor. Its site of synthesis and spectrum of targets imply that this growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. |
| Databáze: | OpenAIRE |
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