Two‐photon excited lasing of Coumarin 307 for lysozyme amyloid fibrils detection
| Autor: | Piotr Hanczyc, Piotr Fita, Czesław Radzewicz, Maciej Procyk |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Amyloid
Materials science Light General Physics and Astronomy 01 natural sciences Two-photon absorption General Biochemistry Genetics and Molecular Biology Fluorescence spectroscopy Antioxidants Protein Structure Secondary law.invention 010309 optics Two-photon excitation microscopy law Coumarins 0103 physical sciences Humans Scattering Radiation General Materials Science Stimulated emission Fluorescent Dyes Photons Ethanol 010401 analytical chemistry General Engineering Neurodegenerative Diseases General Chemistry Laser Fluorescence 0104 chemical sciences Spectrometry Fluorescence Nonlinear Dynamics Biophysics Muramidase Time-resolved spectroscopy Lasing threshold |
| Zdroj: | Journal of Biophotonics |
| DOI: | 10.5281/zenodo.4421349 |
| Popis: | Amyloid fibrils are a well‐recognized hallmark of neurodegeneration. A common approach to detect amyloid fibrils is staining with organic molecules and monitoring optical properties using fluorescence spectroscopy. However, the structural diversity of amyloids necessitates new sensitive methods and probes that can be reliably used to characterize them. Here, Coumarin 307 is applied for lysozyme fibrils detection by observation of laser action in the process of two‐photon excited stimulated emission. It is shown that the lasing threshold and spectrum significantly depend on the adopted structure (α‐helix or β‐sheet) of the lysozyme protein, whereas fluorescence spectrum is insensitive to the protein structure. The applications of coherent stimulated emission light that can be emitted deep inside a scattering medium can be particularly promising for imaging and therapeutic purposes in the neurodegeneration field. Two‐photon excitation with the near‐infrared light, which allows the deepest penetration of tissues, is an important advantage of the method. |
| Databáze: | OpenAIRE |
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