Morpho-biochemical and molecular characterization of two new strains of Aspergillus fumigatus nHF-01 and A. fumigatus PPR-01 producing broad-spectrum antimicrobial compounds
| Autor: | Vivekananda Mandal, Rajsekhar Adhikary, Pulak Kumar Maiti, Sukhendu Mandal |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.disease_cause
DNA Ribosomal Microbiology Aspergillus fumigatus 03 medical and health sciences Anti-Infective Agents Species Specificity Fungal and Bacterial Physiology - Research Paper Phylogenetics Mycology Media Technology medicine Humans DNA Fungal Phylogeny 030304 developmental biology 0303 health sciences biology Strain (chemistry) Phylogenetic tree 030306 microbiology Pathogenic bacteria Pigments Biological Sequence Analysis DNA Antimicrobial biology.organism_classification Culture Media GenBank |
| Zdroj: | Braz J Microbiol |
| ISSN: | 1678-4405 1517-8382 |
| Popis: | The main objective of the study is to characterize two new strains of Aspergillus fumigatus through morphometric, biochemical, molecular methods, and to evaluate their antimicrobial potentiality. The micro-morphotaxonomy, growth, and metabolic behavior of the strains, nHF-01 and PPR-01, were studied in different growth conditions and compared with standard strain. The molecular characterization was done by sequencing the ncrDNA ITS1-5.8S-ITS2 and D1–D2 domains of the nc 28S rDNA region and compared with a secondary structure-based phylogenetic tree. The secretory antimicrobials and pigments were characterized by TLC, UV-Vis, and FT-IR spectroscopy. Both the strains showed distinct growth patterns in different nutritional media and could assimilate a wide range of carbohydrates with distinctive biochemical properties. The molecular characterization revealed the strains, nHF-01 and PPR-01, as Aspergillus fumigatus (GenBank Accession No. MN190286 and MN190284, respectively). It was observed that the strain nHF-01 produces red to brownish pigments having mild antimicrobial activity while the strain PPR-01 does not represent such transformations. The extractable compounds had a significant antimicrobial potentiality against the human pathogenic bacteria. From this analysis, it can be concluded that the nHF-01 and PPR-01 strains are distinct from other A. fumigatus by their unique characters. Large-scale production and detailed molecular elucidation of the antimicrobial compounds may lead to the discovery of new antimicrobial compounds from these strains. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s42770-021-00439-w. |
| Databáze: | OpenAIRE |
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