Cardiac progenitors derived from reprogrammed mesenchymal stem cells contribute to angiomyogenic repair of the infarcted heart
| Autor: | Stephanie Buccini, Khawaja Husnain Haider, Muhammad Ashraf, Shujia Jiang, Rafeeq P.H. Ahmed |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Pluripotency Mesenchymal Physiology Cellular differentiation Induced Pluripotent Stem Cells Myocardial Infarction Neovascularization Physiologic Mice Transgenic 030204 cardiovascular system & hematology Biology Kruppel-Like Factor 4 Mice 03 medical and health sciences 0302 clinical medicine SOX2 Physiology (medical) Animals Myocytes Cardiac Bone marrow Progenitor cell Induced pluripotent stem cell 030304 developmental biology 0303 health sciences Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Heart Reprogramming Original Contribution Embryonic stem cell Cell biology MicroRNAs iPS cells Cell Transformation Neoplastic Heart Function Tests Immunology cardiovascular system Cardiology and Cardiovascular Medicine Adult stem cell |
| Zdroj: | Basic Research in Cardiology |
| ISSN: | 1435-1803 0300-8428 |
| Popis: | The strategy to reprogram somatic stem cells to pluripotency status has provided an alternative source of surrogate ES cells (ESC). We report efficient reprogramming of multipotent bone marrow (BM) mesenchymal stem cells (MSC) to pluripotent status and the resultant MSC derived iPS cells (MiPS) and their derived progenitors effectively repaired the infarcted heart. MSC from young, male, Oct4-GFP transgenic mice were reprogrammed by retroviral transduction with Oct4, Sox2, Klf4, and c-Myc stemness factors. MiPS thus generated displayed characteristics of mouse ESC including morphology, surface antigens, gene and miR expression profiles. MiPS also formed spontaneously beating cardiac progenitors which expressed cardiac specific transcription factors and protein markers including Gata4, Mef2c, Nkx2.5, myosin heavy chain, troponin-I, and troponin-T, and showed ultra structural characteristics typical of cardiomyocytes. Intramyocardial delivery of MiPS (group-2) and their derivative cardiac-like cells (MiPS-CP; group-3) in a mouse model of acute myocardial infarction showed extensive survival and engraftment at 4 weeks with resultant attenuation of infarct size (p |
| Databáze: | OpenAIRE |
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