Detection of Measles Virus-Specific Immunoglobulin M in Dried Venous Blood Samples by Using a Commercial Enzyme Immunoassay
| Autor: | Heath Kelly, Michaela A Riddell, Mike G. Catton, Jennie A. Leydon |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
Adolescent Reference laboratory Antibodies Viral Sensitivity and Specificity Veins Immunoenzyme Techniques Measles virus Morbillivirus Antibody Specificity Virology Digital Video Broadcasting medicine Humans Two sample Blood Specimen Collection Chromatography biology medicine.diagnostic_test Venous blood biology.organism_classification Immunoglobulin M Immunoassay biology.protein Reagent Kits Diagnostic Measles |
| Zdroj: | Journal of Clinical Microbiology. 40:5-9 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.40.1.5-9.2002 |
| Popis: | The optical densities (ODs) of 216 dried venous blood (DVB) samples submitted to the Victorian Infectious Diseases Reference Laboratory as part of enhanced measles surveillance were compared to the ODs of the corresponding serum samples collected at the same time. DVB samples, stored for up to 24 months at 4°C, were tested by the Dade Behring Enzygnost Anti-Measles-Virus/IgM immunoassay. Elution and testing conditions were optimized with the use of spiked DVB samples. The assay showed an overall sensitivity of 90.2% and a specificity of 98.8% for DVB samples compared to the results for serum. When the results were analyzed according to the length of time that the DVB sample had been stored, the assay was 100% sensitive and 97% specific according to the ODs for those samples stored for less than 6 months compared to the results for the corresponding serum samples, with 97.7% agreement between the results for the two sample types. These results demonstrate the potential for the use of DVB samples for the diagnosis of measles in routine diagnostic laboratories. |
| Databáze: | OpenAIRE |
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