NINscope, a versatile miniscope for multi-region circuit investigations
| Autor: | Mario Negrello, J D Bos, Hugo Hoedemaker, Joris E. Coppens, Andres de Groot, Bastijn J.G. van den Boom, Romano M van Genderen, John van Veldhuijzen, Tycho M. Hoogland, Chris I. De Zeeuw, Ingo Willuhn |
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| Přispěvatelé: | Neurosciences, ANS - Systems & Network Neuroscience, Adult Psychiatry, Netherlands Institute for Neuroscience (NIN) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Cerebellum Computer science striatum Stimulation Striatum neuroscience 0302 clinical medicine Cortex (anatomy) Biology (General) 0303 health sciences Behavior Animal General Neuroscience Brain imaging General Medicine Tools and Resources medicine.anatomical_structure Cerebral cortex Medicine cerebral cortex Female Computer hardware Dorsum cerebellum QH301-705.5 Science Optogenetics General Biochemistry Genetics and Molecular Biology Footprint (electronics) 03 medical and health sciences Imaging Three-Dimensional Inertial measurement unit Encoding (memory) medicine Animals Image sensor mouse 030304 developmental biology General Immunology and Microbiology business.industry Led driver Cortex (botany) Mice Inbred C57BL 030104 developmental biology Microscopy Fluorescence Cellular resolution Nerve Net miniscope business Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | eLife, Vol 9 (2020) eLife, 9:e49987. eLife Sciences Publications eLife, 9 eLife |
| ISSN: | 2050-084X |
| Popis: | Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural activity during unrestrained behavior and their open-source versions have helped to distribute them at an affordable cost. Generally, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight, small footprint, open-source miniscope that incorporates a high-sensitivity image sensor, an inertial measurement unit (IMU), and an LED driver for an external optogenetic probe. We highlight the advantages of NINscope by performing the first simultaneous cellular resolution (dual scope) recordings from cerebellum and cerebral cortex in unrestrained mice, revealing that the activity of both regions generally precedes the onset of behavioral acceleration. We further demonstrate the optogenetic stimulation capabilities of NINscope and show that cerebral cortical activity can be driven strongly by cerebellar stimulation. To validate the performance of our miniscope to image from deep-brain regions, we recorded in the dorsal striatum and, using the IMU to assess turning movements, replicate previous studies that show encoding of action space in this subcortical region. Finally, we combine optogenetic stimulation of distinct cortical regions projecting to the dorsal striatum, to probe functional connectivity. In combination with cross-platform control software, NINscope is a versatile addition to the expanding toolbox of open-source miniscopes and will aid multi-region circuit investigations during unrestrained behavior. |
| Databáze: | OpenAIRE |
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