Differences in local immune cell landscape between Q fever and atherosclerotic abdominal aortic aneurysms identified by multiplex immunohistochemistry

Autor: Alexander HJ Staal, Kimberley RG Cortenbach, Teske Schoffelen, Mark AJ Gorris, Lieke L Van der Woude, Anne FM Jansen, Paul Poyck, Robert Jan Van Suylen, Peter C Wever, Chantal P Bleeker-Rovers, Mangala Srinivas, Konnie M Hebeda, Marcel van Deuren, Jos W Van der Meer, Jolanda M De Vries, Roland RJ Van Kimmenade
Rok vydání: 2022
Předmět:
Male
Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2]
T-Lymphocytes
Vascular damage Radboud Institute for Health Sciences [Radboudumc 16]
lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]
Celbiologie en Immunologie
Rare cancers Radboud Institute for Molecular Life Sciences [Radboudumc 9]
Adaptive Immunity
General Biochemistry
Genetics and Molecular Biology
All institutes and research themes of the Radboud University Medical Center
Life Science
Humans
CARUS General
Aged
Inflammation
General Immunology and Microbiology
General Neuroscience
Macrophages
CARUS Algemeen
General Medicine
Middle Aged
Atherosclerosis
Immunohistochemistry
Immunity
Innate
Reconstructive and regenerative medicine Radboud Institute for Health Sciences [Radboudumc 10]
lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]
Cell Biology and Immunology
cardiovascular system
WIAS
Female
Q Fever
Nanomedicine Radboud Institute for Molecular Life Sciences [Radboudumc 19]
Aortic Aneurysm
Abdominal
Zdroj: Elife, 11
eLife, 11
eLife 11 (2022)
ISSN: 2050-084X
Popis: Background:Chronic Q fever is a zoonosis caused by the bacterium Coxiella burnetii which can manifest as infection of an abdominal aortic aneurysm (AAA). Antibiotic therapy often fails, resulting in severe morbidity and high mortality. Whereas previous studies have focused on inflammatory processes in blood, the aim of this study was to investigate local inflammation in aortic tissue.Methods:Multiplex immunohistochemistry was used to investigate local inflammation in Q fever AAAs compared to atherosclerotic AAAs in aorta tissue specimen. Two six-plex panels were used to study both the innate and adaptive immune systems.Results:Q fever AAAs and atherosclerotic AAAs contained similar numbers of CD68+ macrophages and CD3+ T cells. However, in Q fever AAAs, the number of CD68+CD206+ M2 macrophages was increased, while expression of GM-CSF was decreased compared to atherosclerotic AAAs. Furthermore, Q fever AAAs showed an increase in both the number of CD8+ cytotoxic T cells and CD3+CD8-FoxP3+ regulatory T cells. Finally, Q fever AAAs did not contain any well-defined granulomas.Conclusions:These findings demonstrate that despite the presence of pro-inflammatory effector cells, persistent local infection with C. burnetii is associated with an immune-suppressed microenvironment.Funding:This work was supported by SCAN consortium: European Research Area - CardioVascualar Diseases (ERA-CVD) grant [JTC2017-044] and TTW-NWO open technology grant [STW-14716].
Databáze: OpenAIRE
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