Detection of Escherichia cole O157 in French food samples using an immunomagnetic separation method and the VIDAS E. coli O157
| Autor: | C. Mazuy, S. Ray-Gueniot, C. Vernozy-Rozand, A. Meyrand, Y. Richard, S. Boutrand-Loei |
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| Rok vydání: | 1998 |
| Předmět: |
Meat
biology Immunomagnetic Separation Sorbitol-MacConkey agar Beef sample Raw milk medicine.disease_cause biology.organism_classification Immunomagnetic separation Escherichia coli O157 Applied Microbiology and Biotechnology Enterobacteriaceae Microbiology Immunoenzyme Techniques chemistry.chemical_compound fluids and secretions chemistry Cheese medicine Food Microbiology Food science Escherichia coli Cefixime Bacteria medicine.drug |
| Zdroj: | Letters in applied microbiology. 25(6) |
| ISSN: | 0266-8254 |
| Popis: | Two commercially available screening methods, an automated enzyme-linked fluorescent immunoassay (VIDAS E. coli O157) and an immunomagnetic separation followed by culture onto cefixime tellurite sorbitol MacConkey agar (CT-SMAC), were compared for detection of Escherichia coli O157 in naturally and artificially contaminated food samples. A total of 250 naturally contaminated food samples, including raw milk cheeses, poultry, raw sausages and ground beef retail samples, were examined. Four poultry, one raw sausage and one ground beef sample were found to be positive for E. coli O157 by both methods. Of the six positive samples, five were shown to contain sorbitol-positive, O157-positive, H7-negative, motile and non-verotoxin-producing E. coli. |
| Databáze: | OpenAIRE |
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