Tonsil surface epithelium is ideal for monitoring Ki-67 immunohistochemical staining

Autor:	Donald Ming-Tak Ho, Ching-Fen Yang, Hsiang-Ling Ho, Li-Rung Liao, Chih-Yi Hsu
Rok vydání:	2013
Předmět:	Male Quality Control Pathology medicine.medical_specialty Histology Palatine Tonsil Respiratory Mucosa Pathology and Forensic Medicine chemistry.chemical_compound medicine Humans Tissue Distribution Staining and Labeling biology Stomach General Medicine Immunohistochemistry Epithelium Small intestine Staining Ki-67 Antigen medicine.anatomical_structure Antigen retrieval chemistry Ki-67 Tonsil biology.protein Female
Zdroj:	Histopathology. 63:810-816
ISSN:	0309-0167
DOI:	10.1111/his.12228
Popis:	Aims To identify an easily obtainable non-neoplastic tissue that can be used as control material for monitoring optimal Ki-67 immunohistochemical staining. Methods and results Various tissues, including tonsil (60), uterine cervix (31), breast skin (26), oesophagus (15), stomach (15), small intestine (15) and colon (16), were studied in the search for ideal control tissue. Tonsil surface epithelium is superior to other tissues because it displayed an easily recognized Ki-67 staining pattern including high positive (parabasal layer), low positive (intermediate layer) and negative (basal and superficial layers) zones. Moderate to weak staining of the majority of the intermediate cells could serve as a threshold for positive staining. Of the variables potentially affecting staining results that were tested, the pretreatment solution for antigen retrieval had the greatest impact, of which pH 9 EDTA was far better than pH 6 citrate solution. Conclusions Tonsil surface epithelium is a useful control for monitoring Ki-67 staining. Achieving optimal staining results could minimize variations in Ki-67 index due to differences in the staining methods used by different laboratories.
Databáze:	OpenAIRE
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