SIMPoly: A Matlab-Based Image Analysis Tool to Measure Electrospun Polymer Scaffold Fiber Diameter
| Autor: | Ryan Murphy, Leo Banuelos, Ashley Turcott, Evan Dowey, Benjamin Goodwin, Kristen O'Halloran Cardinal |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Scanning electron microscope Polymers 0206 medical engineering Biomedical Engineering polymer fiber measurement Medicine (miscellaneous) Bioengineering electrospun scaffolds 02 engineering and technology Image (mathematics) 03 medical and health sciences Image Processing Computer-Assisted Fiber Polymer scaffold MATLAB electrospinning 030304 developmental biology computer.programming_language chemistry.chemical_classification 0303 health sciences Measure (data warehouse) Tissue Scaffolds Matlab tool Polymer 020601 biomedical engineering Electrospinning Methods Articles chemistry Microscopy Electron Scanning computer Biomedical engineering |
| Zdroj: | Tissue Engineering. Part C, Methods |
| ISSN: | 1937-3392 |
| Popis: | Quantifying fiber diameter is important for characterizing electrospun polymer scaffolds. Many researchers use manual measurement methods, which can be time-consuming and variable. Semi-automated tools exist, but there is room for improvement. The current work used Matlab to develop an image analysis program to quickly and consistently measure fiber diameter in scanning electron micrographs. The new Matlab method, termed “SIMPoly” (Semiautomated Image Measurements of Polymers) was validated by using synthetic images with known fiber size and was found to be accurate. The Matlab method was also applied by three different researchers to scanning electron microscopy (SEM) images of electrospun poly(lactic-co-glycolic acid) (PLGA). Results were compared with the semi-automated DiameterJ method and a manual ImageJ measurement approach, and it was found that the Matlab-based SIMPoly method provided measurements in the expected range and with the least variability between researchers. In conclusion, this work provides and describes SIMPoly, a Matlab-based image analysis method that can simply and accurately measure polymer fiber diameters in SEM images with minimal variation between users. Impact statement This article describes Semiautomated Image Measurements of Polymers (SIMPoly), a novel image analysis tool for measuring polymer fiber diameter in scanning electron micrographs of electrospun scaffolds. The Matlab-based SIMPoly method developed is a semi-automated tool that is quick, easy to use, and absent of significant user bias. This tool can be used to accurately measure electrospun polymer fiber diameters, an important characteristic for characterizing tissue-engineered scaffolds. |
| Databáze: | OpenAIRE |
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