| Autor: |
W.E. Borrias, C.J. Kuhlemeier, N.Tandeau de Marsac, A.M. Castets, C.A.M.J.J. van den Hondel, G. A. Van Arkel |
| Jazyk: |
angličtina |
| Rok vydání: |
1982 |
| Předmět: |
|
| Zdroj: |
Gene, 20(1), 111. Elsevier |
| ISSN: |
0378-1119 |
| Popis: |
A new strategy for molecular cloning in the cyanobacterium Anacystis nidulans R-2 is described. This strategy involved the use of a transposon and was developed for the cloning of a gene encoding methionine biosynthesis. A met::Tn 901 mutant was isolated. Chromosomal DNA fragments were cloned in the Escherichia coli plasmid vector pACYC184. A recombinant plasmid carrying the inactivated met::1n901 gene was selected after transformation to E. coli. The cloned met::Tn901 DNA fragment was used as a probe to select the corresponding A. nidulans R-2 wild-type met gene from a gene library prepared in E. coli, using the newly constructed shuttle cosmid vector pPUC29. When transformed into A. nidulans Met mutants, this cloned gene allowed the mutants to grow prototrophically. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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