Aptamer selection for fishing of palladium ion using graphene oxide-adsorbed nanoparticles
| Autor: | Gwan-Ho Lee, Sang Soo Hah, Eun Jeong Lee, Yea Seul Cho |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Aptamer
Metal ions in aqueous solution Clinical Biochemistry Inorganic chemistry Oxide Pharmaceutical Science chemistry.chemical_element Nanoparticle Nanotechnology Biochemistry Chemistry Techniques Analytical law.invention Ion chemistry.chemical_compound law Drug Discovery Molecular Biology Ions Graphene Organic Chemistry SELEX Aptamer Technique chemistry Molecular Medicine Nanoparticles Graphite Adsorption Palladium |
| Zdroj: | Bioorganicmedicinal chemistry letters. 25(23) |
| ISSN: | 1464-3405 |
| Popis: | A new aptamer selection method using graphene oxide (GO)-adsorbed nanoparticles (GO-adsorbed NPs) was employed for specific fishing of palladium ion. High affinity ssDNA aptamers were isolated through 13 rounds of selection and the capacity of the selected DNA aptamers for palladium ion uptake was measured, clarifying that DNA01 exhibits the highest affinity to palladium ion with a dissociation constant (Kd) of 4.60±1.17 μM. In addition, binding ability of DNA01 to palladium ion was verified against other metal ions, such as Li(+), Cs(+), Mg(2+), and Pt(2+). Results of the present study suggest that future modification of DNA01 may improve palladium ion-binding ability, leading to economic recovery of palladium from water solution. |
| Databáze: | OpenAIRE |
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