Tomato chlorotic leaf distortion virus, a new bipartite begomovirus infecting Solanum lycopersicum and Capsicum chinense in Venezuela

Autor: Francis Geraud-Pouey, Edgloris Marys, Doris Chirinos, K. Zambrano, Gustavo Romay
Přispěvatelé: Instituto Venezolano de Investigaciones Cientificas (IVIC), University of Zulia, Partenaires INRAE, Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), This work was supported in part by grants from FONACIT G-2000001610, convention Cuba-Venezuela (Project: Study and characterization of genetic variability of emerging pest in agricultural ecosystems) to Francis Geraud-Pouey and from CDCHT-UCLA 004-AG-2007 to Karla Zambrano. We are indebted to Dr. Eva de Garcia and Dr. Edith Vargas (IBE, UCV, Venezuela) and Dr. Ariadne Vegas (INIA, Maracay, Venezuela) for assistance with biolistics, and to Mr. Jorge Rivas for assistance with photography
Rok vydání: 2011
Předmět:
Zdroj: Archives of Virology
Archives of Virology, Springer Verlag, 2011, 156 (12), pp.2263-2266. ⟨10.1007/s00705-011-1093-x⟩
ISSN: 1432-8798
0304-8608
Popis: Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; Virus isolate T217L was obtained from a diseased tomato (Solanum lycopersicum) plant showing leaf deformation and chlorotic mottle symptoms near Maracaibo in the state of Zulia, Venezuela. Full-length DNA-A and DNA-B molecules of T217L were cloned and sequenced. The genome organization of T217L was identical to the bipartite genomes of other begomoviruses described from the Americas. Characteristic disease symptoms were reproduced in S. lycopersicum and Capsicum annum plants inoculated using the cloned viral DNA-A and DNA-B components, confirming disease aetiology. A sequence analysis of DNA-A showed that the T217L isolate has the highest sequence identity (84%) with sida yellow mosaic Yucatan virus (SiYMYuV), sida golden mosaic Honduras virus (SiGMHV) and bean dwarf mosaic virus (BDMV) isolates. This is less than the 89% identity in the DNA-A component that has been defined as the threshold value for the demarcation of species in the genus Begomovirus. The molecular data show that isolate T217L belongs to a novel tentative begomovirus species, for which the name tomato chlorotic leaf distortion virus is proposed. TCLDV was also detected in symptomatic C. chinense plants growing near the T217L-infected plant.
Databáze: OpenAIRE
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