Exogeneous diacylglycerols downregulate the activity of Na+-K+ pump in Xenopus laevis oocytes
| Autor: | Naim Akhtar Khan, J.-Ph. Moulinoux, V. Quemener |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
ATPase
Xenopus Biological Transport Active Diaphragm pump In Vitro Techniques Endocytosis Piperazines Ouabain Amiloride Diglycerides Xenopus laevis 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine medicine Animals Na+/K+-ATPase Protein Kinase C Protein kinase C biology Cell Membrane Sodium Cell Biology Isoquinolines Rubidium Oocyte biology.organism_classification Cell biology Kinetics medicine.anatomical_structure Biochemistry Oocytes biology.protein Tetradecanoylphorbol Acetate Female Sodium-Potassium-Exchanging ATPase medicine.drug |
| Zdroj: | Experimental Cell Research. 194:248-251 |
| ISSN: | 0014-4827 |
| Popis: | In this study, cell permeable diacyglycerols, sn-1,2-dioctanoglycerol (DiC8), and sn-1-oleoyl-2-acetylglycerol (OAG) were found to downregulate the activity of Na+-K+ pump in Xenopus laevis oocytes. Both DiC8 and OAG decreased the binding of [3H]ouabain to intact oocytes while phorbol esters did not appreciably influence the same. These diacylglycerols inhibited the amiloride-sensitive 22Na+ influx and ouabain-sensitive 88Rb+ uptake in the oocytes. Furthermore, DiC8 prevented the 22Na+ efflux from the oocytes preloaded with 22Na+. Addition of H-7 to DiC8- and OAG-treated oocytes stimulated the pump activity curtailed by the two latters. The impairment of Na+-K+ pump activity by diacylglycerols suggests that protein kinase C activators may stimulate endocytosis of membrane-coupled Na+-K+ ATPase. |
| Databáze: | OpenAIRE |
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