First report of Pythium aphanidermatum infecting tomato in Egypt and its control using biogenic silver nanoparticles
Autor: | S. M. Lashin, Hesham Mohamed Abouelnasr, Osama M. Darwesh, Ibrahim Elshahawy |
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Rok vydání: | 2023 |
Předmět: |
0106 biological sciences
Pythium aphanidermatum silver nanoparticles (AgNps) Soil Science Plant Science tomato 01 natural sciences Silver nanoparticle SB1-1110 Biogenic substance 010608 biotechnology Botany Soil treatment Fungal morphology biogenic synthesis biology business.industry Pest control food and beverages Plant culture biology.organism_classification Plant disease business Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | Journal of Plant Protection Research, Vol 58, Iss 2, Pp 137-151 (2018) |
ISSN: | 1899-007X 1427-4345 |
DOI: | 10.24425/122929 |
Popis: | In August 2016, tomato plants grown during a hot, wet summer with heavy soil flooding, displaying symptoms of wilting, dead plant, root rot with crown and stem rot, at Beni Suef and Fayoum governorates were examined. A number of 16 fungal isolates were isolated from tomato plants displaying the above symptoms. These isolates were classified as belonging to six species, namely: Alternaria solani, Chaetomium globosum, Fusarium solani, Fusarium oxysporum, Pythium spp. and Rhizoctonia solani. Isolates of Pythium spp. were prevalent and were found to be more pathogenic than the other fungal isolates. This species causes damping-off, root rot, sudden death, stem rot and fruit rot. The pathogen was identified as Pythium aphanidermatum based on morphological, cultural, and molecular characteristics. Biogenic silver nanoparticles (AgNPs) were produced using the F. oxysporum strain and characterized by transmission electron microscopy (TEM). The size of these spherical particles ranged from 10 to 30 nm. In vitro, biogenic AgNPs showed antifungal activity against P. aphanidermatum. In greenhouse and field experiments, AgNPs treatment significantly reduced the incidence of dead tomato plants due to root rot caused by P. aphanidermatum compared to the control. All of the investigated treatments were effective and the treatment of root dipping plus soil drenching was the most effective. To the best of our knowledge, this study describes P. aphanidermatum on tomato in Egypt for the first time. Also, biogenic AgNPs could be used for controlling root rot disease caused by this pathogen. |
Databáze: | OpenAIRE |
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