Improvement of the T-cell response to a non immunogenic peptide by its tandem association with a highly efficient T-helper peptide
| Autor: | Eric Diesis, Jérôme Estaquier, André Capron, Hélène Gras-Masse, Claude Auriault, Pierre-Richard Ridel, C. Mazingue, Franck Rouaix, André Tartar, M. Marguerite |
|---|---|
| Přispěvatelé: | Deleage, Gilbert |
| Rok vydání: | 1994 |
| Předmět: |
Male
Cellular immunity Antigenicity T-Lymphocytes Molecular Sequence Data Peptide Biology Lymphocyte Activation Gene Products nef Epitope Epitopes Adjuvants Immunologic Antigen [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals Amino Acid Sequence Pharmacology chemistry.chemical_classification Immunogenicity Rational design Schistosoma mansoni biology.organism_classification Molecular biology Rats Biochemistry chemistry Rats Inbred Lew Antigens Helminth Peptides |
| Zdroj: | Immunopharmacology. 28:215-222 |
| ISSN: | 0162-3109 |
| DOI: | 10.1016/0162-3109(94)90057-4 |
| Popis: | The 45-69 peptide, an helper T-cell epitope derived from the HIV nef protein is strongly immunogenic. A T-cell proliferative response was observed following immunization of Lou/M rats with 45-69 peptide administered in low dose and without any adjuvant. It is already known that the T-cell response to the 115-131 peptide of Sm28GST antigen, a protein of the parasite Schistosoma mansoni, requires the presence of a carrier of the use of peptidic constructs. We demonstrate here that a T-cell response against the 115-131 peptide can be obtained in the absence of adjuvant using peptidic constructs (115-45 and 45-115 peptides) resulting from tandem synthesis of 115-131 and 45-69 peptides. A covalent association of both peptides is necessary, since the coinjection of 45-69 and 115-131 peptides is not sufficient to induce a detectable anti-115-131 T-cell response. The mutual orientation between the respective tandem peptides (45-115 and 115-45) is critical for the T-cell response. These peptidic constructs possess distinct properties of antigenicity and immunogenicity but both allowed to reveal the existence of a specific T-cell response normally undetectable using 115-131 peptide alone. This immunopharmacological approach should be useful in the rational design and construction of vaccines.The 45-69 peptide, an helper T-cell epitope derived from the HIV nef protein is strongly immunogenic. A T-cell proliferative response was observed following immunization of Lou/M rats with 45-69 peptide administered in low dose and without any adjuvant. It is already known that the T-cell response to the 115-131 peptide of Sm28GST antigen, a protein of the parasite Schistosoma mansoni, requires the presence of a carrier of the use of peptidic constructs. We demonstrate here that a T-cell response against the 115-131 peptide can be obtained in the absence of adjuvant using peptidic constructs (115-45 and 45-115 peptides) resulting from tandem synthesis of 115-131 and 45-69 peptides. A covalent association of both peptides is necessary, since the coinjection of 45-69 and 115-131 peptides is not sufficient to induce a detectable anti-115-131 T-cell response. The mutual orientation between the respective tandem peptides (45-115 and 115-45) is critical for the T-cell response. These peptidic constructs possess distinct properties of antigenicity and immunogenicity but both allowed to reveal the existence of a specific T-cell response normally undetectable using 115-131 peptide alone. This immunopharmacological approach should be useful in the rational design and construction of vaccines. |
| Databáze: | OpenAIRE |
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