Restricted expression of HIV1 in human astrocytes: molecular basis for viral persistence in the CNS
| Autor: | C. Möller, V. Erfle, Andrea Kleinschmidt, Ruth Brack-Werner, M. Neumann |
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| Rok vydání: | 1994 |
| Předmět: |
Central Nervous System
Immunology HIV Core Protein p24 Gene Expression Biology Virus Replication Gene Products nef Virus Cell Line chemistry.chemical_compound Virology medicine Humans RNA Messenger nef Gene Products Human Immunodeficiency Virus Tumor Necrosis Factor-alpha Sodium butyrate Phenotype Butyrates medicine.anatomical_structure chemistry Viral replication Cell culture Astrocytes HIV-1 Butyric Acid RNA Viral Tetradecanoylphorbol Acetate Neuroglia Tumor necrosis factor alpha Astrocyte |
| Zdroj: | Research in Virology. 145:147-153 |
| ISSN: | 0923-2516 |
| Popis: | Besides macrophages and microglial cells, cells of astroglial origin are thought to be targets of HIV1 in the brain. HIV1 infection of astroglial cells results in restricted production of the virus. To analyse the molecular basis of this restricted infection phenotype, we established a chronically HIV1-infected low-producer astrocytoma cell line. These cells show only low levels of mRNA encoding structural proteins, due to a cell-determined blockage in the Rev/RRE regulatory axis. The low-producer state could not be overcome by treatment with known stimulators of virus expression such as phorbol ester, (12-O-tetradecanoylphorbol-13-acetate), tumour necrosis factor alpha or sodium butyrate. This indicates that the molecular mechanisms involved in restricting virus production in astroglial cells differ from those in latently infected T cells and monocytes. |
| Databáze: | OpenAIRE |
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