Morphine induces the differentiation of T helper cells to Th2 effector cells via the PKC-θ–GATA3 pathway
Autor: | Tieliang Ma, Daoyun Lei, Chao Han, Hong Zhou, Shitong Li, Songhui Xie, Shuang Wen, Li Liu, Lianping He |
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Rok vydání: | 2020 |
Předmět: |
Male
0301 basic medicine Naive T cell Narcotic Antagonists Cellular differentiation Immunology Cell GATA3 Transcription Factor Flow cytometry Interferon-gamma 03 medical and health sciences 0302 clinical medicine medicine Animals Immunology and Allergy Pyrroles Electrophoretic mobility shift assay Transcription factor Pharmacology Morphine medicine.diagnostic_test Chemistry GATA3 Cell Differentiation T-Lymphocytes Helper-Inducer Naltrexone Cell biology Analgesics Opioid Mice Inbred C57BL 030104 developmental biology Real-time polymerase chain reaction medicine.anatomical_structure Protein Kinase C-theta 030220 oncology & carcinogenesis Quinazolines Interleukin-4 |
Zdroj: | International Immunopharmacology. 80:106133 |
ISSN: | 1567-5769 |
Popis: | Background T help 2 (Th2) cell differentiation by morphine has been verified. However, the underlying mechanism of morphine induces Th2 cell differentiation remains elusive. The aim of the present study was to explore the possible basis of morphine induced Th2 cell differentiation. Methods Flow cytometry analysis was used to detect the content of T help 1(Th1) cell and Th2 cell. Enzyme linked immunosorbent assay (ELISA) was performed to determine the levels of IL-4 and IFN-γ. Real-time quantitative polymerase chain reaction, electrophoretic mobility shift assay and Western blotting was conducted in this study. Results Th2 cell subset and IL-4 level were elevated in morphine induced naive T cells. Pathway determining found the protein phosphorylation level of PKC-θ and the expression and activity of the transcription factor GATA3 was also enhanced in the naive T cells challenged by morphine. Moreover, inhibitor of morphine(naltrexone) or PKC-θ(AEB071) can reverse morphine-induced Th2 cell differentiation. Conclusion These results suggested that morphine induce naive T cell differentiation to Th2 cells via the PKC-θ/GATA3 signal pathway. |
Databáze: | OpenAIRE |
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