Periostin as a key molecule defining desmoplastic environment in colorectal cancer
| Autor: | Takahiro Sueyama, Hideki Ueno, Yoshiki Kajiwara, Kazuo Hase, Eiji Shinto, Satsuki Mochizuki, Hideyuki Shimazaki |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Oncology medicine.medical_specialty Colorectal cancer Periostin Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Cancer-Associated Fibroblasts Western blot Stroma Internal medicine Biomarkers Tumor Tumor Microenvironment medicine Humans Molecular Biology Aged Retrospective Studies Aged 80 and over Messenger RNA biology medicine.diagnostic_test business.industry Cell Biology General Medicine Middle Aged Prognosis medicine.disease Up-Regulation Gene Expression Regulation Neoplastic 030104 developmental biology 030220 oncology & carcinogenesis Cohort biology.protein Immunohistochemistry Female Stromal Cells Antibody Colorectal Neoplasms business Cell Adhesion Molecules |
| Zdroj: | Virchows Archiv. 478:865-874 |
| ISSN: | 1432-2307 0945-6317 |
| Popis: | Categorizing desmoplastic reaction (DR) based on the histological findings of cancer-associated fibroblasts is shown to be a promising novel method to predict prognosis of patients with colorectal cancer (CRC). Periostin (POSTN) in cancer-associated stroma is reportedly associated with poor clinical outcomes. Immunohistochemical staining with an anti-POSTN antibody was performed in 73 patients with pStage III CRC (cohort 1). In addition, to evaluate mRNA and protein expression levels of POSTN, we analyzed paired normal and invasive cancer frozen specimens by quantitative real-time polymerase chain reaction and western blot analysis in 41 patients (cohort 2). In cohort 1, according to the DR categorization, 18, 22, and 33 patients were classified as immature, intermediate, and mature, respectively. High immunoreactivity of POSTN was observed 100%, 68.2%, and 27.3%, respectively (p |
| Databáze: | OpenAIRE |
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