An in vitro screening method for probiotics with antidepressant-like effect using the enterochromaffin cell model
| Autor: | Mengshu Xu, Jianxin Zhao, Zhu Huiyue, Wei Chen, Wang Gang, Qinming Kong, Peijun Tian, Hao Zhang, Renying Zou |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Serotonin Biology Pharmacology Tryptophan Hydroxylase Gene Expression Regulation Enzymologic 03 medical and health sciences Mice 0302 clinical medicine In vivo Stress Physiological Enterochromaffin Cells Animals Humans Secretion Swimming Messenger RNA Principal Component Analysis TPH1 Bacteria Probiotics General Medicine In vitro Antidepressive Agents Mice Inbred C57BL 030104 developmental biology Enterochromaffin cell 030217 neurology & neurosurgery Function (biology) Food Science Behavioural despair test |
| Zdroj: | Foodfunction. 12(2) |
| ISSN: | 2042-650X |
| Popis: | Certain probiotics can regulate the host's neurobehavioral function through the microbiota-gut-brain axis. However, screening these probiotics is mainly carried out in animal models, and is costly and inefficient. Herein, a putative enterochromaffin cell line (RIN14B) was used as an in vitro pre-screening model; 30 bacterial strains were tested for bacteria-stimulated tryptophan hydroxylase 1 gene (Tph1) expression and 5-hydroxytryptophan/5-hydroxytryptamine secretion. All strains were further validated for their neurobehavioral effects in chronic stress-induced depressive mice. Using partial least squares (PLS) modeling of in vitro and in vivo datasets, we found that the level of Tph1 mRNA in RIN14B significantly correlated with the performance of a forced swim test and sucrose preference test, and serum corticosterone level in chronically stressed mice. Four strains were identified as the best candidates among 30 strains using principal component analysis on all in vivo measures, and unsurprisingly, three of them could enhance Tph1 expression in RIN14B, which further proved that the RIN14B-based screening method (especially the detection of bacteria-stimulated Tph1 mRNA) has good predictive validity and screening efficiency for the strain's antidepressant-like capacity. Collectively, this study provides a novel in vitro method for screening probiotics (or other related bioproducts) with antidepressant-like potential. |
| Databáze: | OpenAIRE |
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