A Mouse Acrosomal Cortical Matrix Protein, MC41, Has ZP2-Binding Activity and Forms a Complex with a 75-kDa Serine Protease
| Autor: | Tadasuke Oh-oka, Kiyotaka Toshimori, Kazuya Yoshinaga, Ichiro Tanii |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Proteases Octoxynol serine protease zona pellucida Blotting Western Detergents Immunoblotting Acrosome reaction acrosome reaction Receptors Cell Surface acrosomal matrix Biology Acrosomal matrix Zona Pellucida Glycoproteins sperm–zona interaction Mice subdomain structure secondary binding medicine Animals Far-western blotting Antigens Microscopy Immunoelectron Zona pellucida Acrosome Molecular Biology mouse Serine protease Mice Inbred ICR Membrane Glycoproteins Egg Proteins Serine Endopeptidases Antibodies Monoclonal Cell Biology Immunohistochemistry Spermatozoa Sperm Molecular biology Protein Structure Tertiary Cell biology Microscopy Electron medicine.anatomical_structure fertilization biology.protein Female Protein Binding Developmental Biology |
| Zdroj: | Developmental Biology. 238:332-341 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.2001.0380 |
| Popis: | Sperm with a large acrosome such as that of guinea pigs and hamsters have a subdomain structure in the anterior acrosome, but the mouse acrosome looks homogeneous and its matrix has not been precisely analyzed. The intra-acrosomal protein MC41 is localized in the cortical region of the mouse anterior acrosome, suggesting a subdomain structure in the mouse acrosome. Thus, the present study was undertaken to analyze the mouse acrosomal matrix using an anti-MC41 antibody. When mouse sperm were treated with 2% Triton X-100, Triton-insoluble matrix components remained in the acrosomal cortical region. Immunogold for MC41 labeled the Triton X-100 and high-salt-insoluble matrix components, demonstrating that MC41 is a subdomain-specific acrosomal matrix protein. We further examined interactions of MC41 with acrosomal proteases and zona proteins. A serine protease of 75 kDa was associated with MC41 under low-salt conditions, presumably forming a complex. Far Western blotting technique indicated that MC41 bound to both ZP2 and ZP2 f in the presence of high-salt-soluble sperm proteins. In acrosome-reacting sperm, MC41 was present on the hybrid vesicles formed by the fusion of the plasma and outer acrosomal membranes. Presumably, MC41 has a significant role in secondary sperm–zona binding during the acrosomal reaction. |
| Databáze: | OpenAIRE |
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