Monocyte-derived fibrocytes elimination had little contribution on liver fibrosis
| Autor: | Kenichi Nakamura, Kazunori Kusumoto, Hisayoshi Iwakiri, Satoru Hasuike, Mai Tsuchimochi, Fumiyo Toyoshima, Kotaro Shide, Yuuka Takaishi, Ayako Kamiunten, Takuro Kameda, Kazuya Shimoda, Tadashi Miike, Yoshinori Ozono, Kenji Nagata, Akira Sawaguchi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Pathology
medicine.medical_specialty Time Factors Liver Cirrhosis Experimental digestive system Peripheral blood mononuclear cell Collagen Type I Monocytes 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Fibrosis parasitic diseases Fibrocyte medicine Animals Carbon Tetrachloride Sirius Red Kidney Hepatology business.industry Monocyte Gastroenterology medicine.disease Up-Regulation Staining Mice Inbred C57BL medicine.anatomical_structure Liver chemistry 030220 oncology & carcinogenesis Disease Progression Hepatic stellate cell Female 030211 gastroenterology & hepatology Chemical and Drug Induced Liver Injury Clodronic Acid business |
| Zdroj: | Hepatobiliary & Pancreatic Diseases International. 18:348-353 |
| ISSN: | 1499-3872 |
| DOI: | 10.1016/j.hbpd.2019.02.002 |
| Popis: | Background Monocyte-derived fibrocytes play an important role in the progression of fibrosis in the skin, lungs, heart and kidney. However, the contribution of fibrocytes to liver fibrosis is unclear. The aim of this study was to investigate whether fibrocytes contributed to fibrosis progression in the livers of carbon tetrachloride (CCl4)-treated mice. Methods C57BL/6J mice were divided into 4 groups: normal control group, CCl4-treated group, CCl4 + control liposome-treated group, and CCl4 + clodronate liposome-treated group. For the elimination of systemic monocyte and monocyte-derived fibrocyte, one group was treated with clodronate liposome, and another group with control liposome as a control. After 4 weeks of treatment, hepatic mononuclear cells were subjected to immunofluorescent (IF) staining and fluorescence-activated cell sorter (FACS) analysis to detect fibrocytes. Measurement of collagen-positive Sirius red stained area and collagen-I mRNA expression in the liver were performed to evaluate the degree of liver fibrosis quantitatively. Results In the liver of the CCl4-treated and CCl4 + control liposome-treated groups, the number of fibrocytes, the area positive for Sirius red staining and collagen-I mRNA expression significantly increased compared with those in the normal control group. In the liver of the CCl4 + clodronate liposome-treated group, few fibrocytes was observed as in the normal control group, but Sirius red staining positive area and collagen-I mRNA expression were increased and equivalent to the CCl4-treated and CCl4 + control liposome-treated groups. Conclusion Monocyte-derived fibrocytes play a minimal role in CCl4-induced liver fibrosis. Cells other than fibrocytes such as hepatic stellate cells play a central role in liver fibrosis. |
| Databáze: | OpenAIRE |
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