Promoter Methylation and mRNA Expression of Response Gene to Complement 32 in Breast Carcinoma
| Autor: | Firoozeh Rafighdoost, Mohammad Hashemi, Ebrahim Eskandari-Nasab |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Messenger RNA Article Subject Epidemiology lcsh:R Public Health Environmental and Occupational Health lcsh:Medicine Methylation Biology medicine.disease_cause medicine.disease Molecular biology 03 medical and health sciences 030104 developmental biology Real-time polymerase chain reaction Breast cancer Downregulation and upregulation Genetics medicine Cancer research Carcinogenesis Breast carcinoma Gene Research Article |
| Zdroj: | Journal of Cancer Epidemiology Journal of Cancer Epidemiology, Vol 2016 (2016) |
| ISSN: | 1687-8558 |
| DOI: | 10.1155/2016/7680523 |
| Popis: | Background. Response gene to complement 32 (RGC32), induced by activation of complements, has been characterized as a cell cycle regulator; however, its role in carcinogenesis is still controversial. In the present study we comparedRGC32promoter methylation patterns and mRNA expression in breast cancerous tissues and adjacent normal tissues.Materials and Methods. Sixty-three breast cancer tissues and 63 adjacent nonneoplastic tissues were included in our study.Design. Nested methylation-specific polymerase chain reaction (Nested-MSP) and quantitative PCR (qPCR) were used to determineRGC32promoter methylation status and its mRNA expression levels, respectively.Results. RGC32 methylation pattern was not different between breast cancerous tissue and adjacent nonneoplastic tissue (OR = 2.30, 95% CI = 0.95–5.54). However, qPCR analysis displayed higher levels ofRGC32mRNA in breast cancerous tissues than in noncancerous tissues (1.073 versus 0.959;P=0.001), irrespective of the promoter methylation status. The expression levels and promoter methylation ofRGC32were not correlated with any of patients’ clinical characteristics (P>0.05).Conclusion. Our findings confirmed upregulation of RGC32 in breast cancerous tumors, but it was not associated with promoter methylation patterns. |
| Databáze: | OpenAIRE |
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