Genetic Diversity and Origins of the Homoploid-Type Hybrid Phytophthora ×alni
| Autor: | Aguayo, Jaime, Halkett, Fabien, Husson, Claude, Nagy, Zoltán Á., Szigethy, András, Bakonyi, József, Frey, Pascal, Marcais, Benoit, Master, E. |
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| Přispěvatelé: | Unité de Mycologie, Laboratoire de la Santé des Végétaux, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Interactions Arbres-Microorganismes (IAM), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Plant Protection Institute, Lorraine Region, FEDER, OTKA K61107, European Cooperation in Science and Technology (COST) FP0801 STSM, Agence Nationale de la Recherche (ANR) ANR 07-BDIV-003 ANR-11-BSV7-0007 ANR-11-LABX-0002-01, Marcais, Benoit, Master, E., Laboratoire de la Santé des Végétaux, Unité de Mycologie (LSV Nancy), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Chercheur indépendant |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MICROSATELLITE MARKERS PLANT-PATHOGENS MULTIVARIATE-ANALYSIS POPULATION-GENETICS ALDER PHYTOPHTHORAS BLACK ALDER R-PACKAGE HYBRIDIZATION SPECIATION AGGRESSIVENESS [SDV]Life Sciences [q-bio] Population genetics Alnus Applied Microbiology and Biotechnology Plant Microbiology genetique des populations Phylogeny polyploidy Genetics education.field_of_study Ecology biology Europe génération parentale marqueur microsatellite Genetic structure Microsatellite Phytophthora Biotechnology Genotype Population Phytophthora alni DNA Mitochondrial 03 medical and health sciences Polyploid plante pathogène polyploïdie education Plant Diseases Genetic diversity hybrid Genetic Variation 15. Life on land biology.organism_classification hybride 030104 developmental biology Hybridization Genetic phytophthora alni microsatellite repeats Food Science |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2016, 82 (24), pp.7142-7153. ⟨10.1128/AEM.02221-16⟩ Applied and Environmental Microbiology 24 (82), 7142-7153. (2016) |
| ISSN: | 0099-2240 1098-5336 |
| Popis: | Assessing the process that gives rise to hybrid pathogens is central to understanding the evolution of emerging plant diseases. Phytophthora ×alni , a pathogen of alder, results from the homoploid hybridization of two related species, Phytophthora uniformis and Phytophthora × multiformis . Describing the genetic characteristics of P . × alni should help us understand how reproductive mechanisms and historical processes shaped the population structure of this emerging hybrid pathogen. The population genetic structure of P . × alni and the relationship with its parental species were investigated using 12 microsatellites and one mitochondrial DNA (mtDNA) marker on a European collection of 379 isolates. Populations of P . × alni were dominated by one multilocus genotype (MLG). The frequency of this dominant MLG increased after the disease emergence together with a decline in diversity, suggesting that it was favored by a genetic mechanism such as drift or selection. Combined microsatellite and mtDNA results confirmed that P . × alni originated from multiple hybridization events that involved different genotypes of the progenitors. Our detailed analyses point to a geographic structure that mirrors that observed for P. uniformis in Europe. The study provides more insights on the contribution of P. uniformis , an invasive species in Europe, to the emergence of Phytophthora -induced alder decline. IMPORTANCE Our study describes an original approach to assess the population genetics of polyploid organisms using microsatellite markers. By studying the parental subgenomes present in the interspecific hybrid P. ×alni , we were able to assess the geographical and temporal structure of European populations of the hybrid, shedding new light on the evolution of an emerging plant pathogen. In turn, the study of the parental subgenomes permitted us to assess some genetic characteristics of the parental species of P. ×alni , P. uniformis , and P . × multiformis , which are seldom sampled in nature. The subgenomes found in P. ×alni represent a picture of the “fossilized” diversity of the parental species. |
| Databáze: | OpenAIRE |
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