Skin vaccination with live virus vectored microneedle arrays induce long lived CD8+ T cell memory
| Autor: | Linda S. Klavinskis, Pablo D. Becker, Sung-Yun Kwon, Catherine Hervouet, Gavin M. Mason |
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| Rok vydání: | 2015 |
| Předmět: |
Injections
Intradermal T cell CD8-Positive T-Lymphocytes Biology Viral vector Drug Delivery Systems Antigen T-Lymphocyte Subsets medicine Animals Cytotoxic T cell Interleukin-7 receptor Skin AIDS Vaccines Vaccines Synthetic General Veterinary General Immunology and Microbiology T-cell receptor Public Health Environmental and Occupational Health Virology Mice Inbred C57BL Infectious Diseases medicine.anatomical_structure Immunology Molecular Medicine Immunologic Memory Memory T cell CD8 |
| Zdroj: | Vaccine. 33:4691-4698 |
| ISSN: | 0264-410X |
| Popis: | A simple dissolvable microneedle array (MA) platform has emerged as a promising technology for vaccine delivery, due to needle-free injection with a formulation that preserves the immunogenicity of live viral vectored vaccines dried in the MA matrix. While recent studies have focused largely on design parameters optimized to induce primary CD8(+) T cell responses, the hallmark of a vaccine is synonymous with engendering long-lasting memory. Here, we address the capacity of dried MA vaccination to programme phenotypic markers indicative of effector/memory CD8(+) T cell subsets and also responsiveness to recall antigen benchmarked against conventional intradermal (ID) injection. We show that despite a slightly lower frequency of dividing T cell receptor transgenic CD8(+) T cells in secondary lymphoid tissue at an early time point, the absolute number of CD8(+) T cells expressing an effector memory (CD62L(-)CD127(+)) and central memory (CD62L(+)CD127(+)) phenotype during peak expansion were comparable after MA and ID vaccination with a recombinant human adenovirus type 5 vector (AdHu5) encoding HIV-1 gag. Similarly, both vaccination routes generated CD8(+) memory T cell subsets detected in draining LNs for at least two years post-vaccination capable of responding to secondary antigen. These data suggest that CD8(+) T cell effector/memory generation and long-term memory is largely unaffected by physical differences in vaccine delivery to the skin via dried MA or ID suspension. |
| Databáze: | OpenAIRE |
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