Highly Immunogenic and Protective Recombinant Vaccine Candidate Expressed in Transgenic Plants
| Autor: | Patricia Obregon, Alessandra Prada, Julian K.-C. Ma, Daniel Chargelegue, Neil F. Fairweather, Pascal M. W. Drake |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Antigen-Antibody Complex
medicine.medical_treatment Immunology Blotting Western chemical and pharmacologic phenomena Enzyme-Linked Immunosorbent Assay Biology Immune complex formation Microbiology Cell Line Mice Antigen Tetanus Toxin medicine Vaccines DNA Animals Mice Inbred BALB C Vaccines Synthetic Immunogenicity Antibodies Monoclonal Plants Genetically Modified Virology Fusion protein Immune complex Peptide Fragments Infectious Diseases Microbial Immunity and Vaccines biology.protein Parasitology Female Antibody Adjuvant |
| Popis: | Vaccine development has been hampered by difficulties in developing new and safe adjuvants, so alternative technologies that offer new avenues forward are urgently needed. The goal of this study was to express a monoclonal recombinant immune complex in a transgenic plant. A recombinant protein consisting of a tetanus toxin C fragment-specific monoclonal antibody fused with the tetanus toxin C fragment was designed and expressed. Immune complex formation occurred between individual fusion proteins to form immune complex-like aggregates that bound C1q and FcγRIIa receptor and could be targeted to antigen-presenting cells. Unlike antigen alone, the recombinant immune fusion complexes were highly immunogenic in mice and did not require coadministration of an adjuvant (when injected subcutaneously). Indeed, these complexes elicited antibody titers that were more than 10,000 times higher than those observed in animals immunized with the antigen alone. Furthermore, animals immunized with only 1 μg of recombinant immune complex without adjuvant were fully protected against lethal challenge. This the first report on the use of a genetic fusion between antigen and antibody to ensure an optimal expression ratio between the two moieties and to obtain fully functional recombinant immune complexes as a new vaccine model. |
| Databáze: | OpenAIRE |
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