Automated analysis of fluorescence in situ hybridization on fixed, paraffin-embedded whole tissue sections in B-cell lymphoma
| Autor: | Kaaren K. Reichard, Ayumi I. Corn, M. Kathryn Foucar, John Hozier, Bryan Hall |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Lymphoma B-Cell Tissue Fixation Follicular lymphoma In situ hybridization Lymphoma Mantle-Cell Biology Translocation Genetic Pathology and Forensic Medicine hemic and lymphatic diseases medicine Image Processing Computer-Assisted Neoplasm Humans B-cell lymphoma Lymphoma Follicular In Situ Hybridization Fluorescence Chromosomes Human Pair 14 Paraffin Embedding medicine.diagnostic_test Chromosomes Human Pair 11 medicine.disease Prognosis Molecular biology Burkitt Lymphoma Lymphoma Mantle cell lymphoma Lymph Nodes Clone (B-cell biology) Chromosomes Human Pair 18 Fluorescence in situ hybridization Chromosomes Human Pair 8 |
| Zdroj: | Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 19(8) |
| ISSN: | 0893-3952 |
| Popis: | Certain recurrent cytogenetic abnormalities are diagnostic of a specific neoplasm and may portend prognosis. As conventional cytogenetics may not reveal a neoplastic clone, and unfixed material for fluorescence in situ hybridization may be unavailable, performing fluorescence in situ hybridization on fixed tissues is diagnostically and prognostically valuable. Manual interpretation of fluorescence in situ hybridization signals may be difficult on paraffin-embedded tissue sections due to truncated nuclei. Therefore, we investigated the use of an automated image acquisition and analysis system (MetaSystems) for interpretation of fluorescence in situ hybridization signals in tissue sections from dual fusion translocation probes. Three probe sets were analyzed on archival specimens with a confirmed diagnosis of mantle cell lymphoma, follicular lymphoma or Burkitt lymphoma. 100% of mantle cell lymphomas (7/7) were positive for t(11;14), 91% of follicular lymphomas (10/11) for t(14;18) and 100% of Burkitt lymphomas (9/9) for t(8;14). Successful hybridization was achieved using various tissue fixatives and fluorescence in situ hybridization interpretation was blinded with respect to the underlying diagnosis. Based on these results, automated analysis of fluorescence in situ hybridization on fixed tissues is accurate and valuable in the evaluation of B-cell lymphoma, and may provide pertinent diagnostic and prognostic information. |
| Databáze: | OpenAIRE |
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