Direct interaction between DNMT1 and G9a coordinates DNA and histone methylation during replication
| Autor: | Hang Gyeong Chin, Andrea Smallwood, Sriharsa Pradhan, Adam R. Karpf, Pierre-Olivier Estève, George R. Feehery, Michael Carey, Omkaram Gangisetty |
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| Rok vydání: | 2006 |
| Předmět: |
DNA Replication
Biology environment and public health Chromatin remodeling Histones Jurkat Cells Heterochromatin Chlorocebus aethiops DNA Ribosomal Spacer Histone methylation Genetics Animals Humans Histone code DNA (Cytosine-5-)-Methyltransferases Protein Methyltransferases RNA-Directed DNA Methylation Epigenomics Models Genetic urogenital system Histone-Lysine N-Methyltransferase Methyltransferases DNA Methylation HCT116 Cells Molecular biology Chromatin Repressor Proteins Protein Transport Histone methyltransferase COS Cells embryonic structures DNA methylation Histone Methyltransferases HeLa Cells Protein Binding Research Paper Developmental Biology |
| Zdroj: | Genes & Development. 20:3089-3103 |
| ISSN: | 1549-5477 0890-9369 |
| DOI: | 10.1101/gad.1463706 |
| Popis: | Chromatin methylation is necessary for stable repression of gene expression during mammalian development. During cell division, DNMT1 maintains the DNA methylation pattern of the newly synthesized daughter strand, while G9a methylates H3K9. Here, DNMT1 is shown to directly bind G9a both in vivo and in vitro and to colocalize in the nucleus during DNA replication. The complex of DNMT1 and G9a colocalizes with dimethylated H3K9 (H3K9me2) at replication foci. Similarly, another H3K9 histone methyltransferase, SUV39H1, colocalizes with DNMT1 on heterochromatic regions of the nucleoli exclusively before cell division. Both DNMT1 and G9a are loaded onto the chromatin simultaneously in a ternary complex with loading factor PCNA during chromatin replication. Small interfering RNA (siRNA) knockdown of DNMT1 impairs DNA methylation, G9a loading, and H3K9 methylation on chromatin and rDNA repeats, confirming DNMT1 as the primary loading factor. Additionally, the complex of DNMT1 and G9a led to enhanced DNA and histone methylation of in vitro assembled chromatin substrates. Thus, direct cooperation between DNMT1 and G9a provides a mechanism of coordinated DNA and H3K9 methylation during cell division. |
| Databáze: | OpenAIRE |
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