Synchronizing temperatures and rRNA metabolism in Tetrahymena pyriformis
| Autor: | Bettina Hemmerich, Werner A. Eckert, Walter Kaffenberger |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Time Factors
Physiology Kinetics Uridine Triphosphate Biology Biochemistry Adenosine Triphosphate Transcription (biology) Microsomes medicine Animals Nucleotide Molecular Biology Uridine chemistry.chemical_classification Cell Nucleus Tetrahymena pyriformis Temperature General Medicine Ribosomal RNA Adenosine chemistry Cytoplasm RNA Ribosomal Electrophoresis Polyacrylamide Gel Intracellular medicine.drug |
| Zdroj: | Comparative biochemistry and physiology. B, Comparative biochemistry. 64(2) |
| ISSN: | 0305-0491 |
| Popis: | 1. 1. In Tetrahymena pyriformis pre-rRNA is synthesized and efficiently processed and translocated into the cytoplasm at both a supraoptimal (34°C) and a suboptimal (8°C) synchronizing temperature. 2. 2. At the heat shock temperature (34°C) no substantial differences in the kinetics of the main intracellular events of rRNA-metabolism compared to the optimal growth temperature (28°C) were found. 3. 3. The high temperature, however, induces a strong retardation of uptake of [ 3 H]adenosine into the cells and a reduction of the cellular ATP pool size. 4. 4. At the cold shock temperature (8°C) the rates of transcription and nucleocytoplasmic transport of rRNA as well as of nucleotide pool equilibration are reduced to a similar extent (25–30% of the optimal rates at 28°C). 5. 5. The results are discussed and compared with the effects of sub- and supraoptimal temperatures on rRNA synthesis and processing in mammalian cells. |
| Databáze: | OpenAIRE |
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