β-caryophyllene, a dietary cannabinoid, complexed with β-cyclodextrin produced anti-hyperalgesic effect involving the inhibition of Fos expression in superficial dorsal horn
| Autor: | Renan G. Brito, Jullyana S.S. Quintans, Nicole K. Brogden, Mairim Russo Serafini, Flavio Machado de Souza Carvalho, Gabriel Francisco da Silva, Kathleen A. Sluka, Priscila L. Santos, Paula dos Passos Menezes, Adriano Antunes de Souza Araújo, Lucindo José Quintans-Júnior |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Spinal Cord Dorsal Horn medicine.medical_specialty medicine.medical_treatment Beta-Cyclodextrins General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals General Pharmacology Toxicology and Pharmaceutics Saline Polycyclic Sesquiterpenes Cannabinoids business.industry Anti-Inflammatory Agents Non-Steroidal beta-Cyclodextrins Chronic pain General Medicine medicine.disease Spinal cord Lumbar Spinal Cord Treatment Outcome 030104 developmental biology Endocrinology medicine.anatomical_structure Gene Expression Regulation Hyperalgesia Anesthesia Drug Therapy Combination Cannabinoid medicine.symptom business Proto-Oncogene Proteins c-fos Sesquiterpenes 030217 neurology & neurosurgery |
| Zdroj: | Life Sciences. 149:34-41 |
| ISSN: | 0024-3205 |
| Popis: | Evaluate the anti-hyperalgesic effect of the complex containing β-caryophyllene (βCP) and β-cyclodextrin (βCD) in a non-inflammatory chronic muscle pain mice model and investigated its action on superficial dorsal horn of the lumbar spinal cord.The βCP-βCD complex were prepared and characterized through the DSC, TG/DTG, FTIR, XRD and SEM. The model of chronic muscle pain was induced by two injections of pH4.0 saline (20μL) into left gastrocnemius 5days apart. After confirming hyperalgesia, male mice were treated with βCP-βCD (10 or 20mg/kg; p.o.) or vehicle (saline 0.9%, p.o.) daily for 9days. 1h after, the mechanical hyperalgesia, muscle withdrawal thresholds and motor performance were evaluated. To evaluate the βCP-βCD action on spinal cord, animals induced with chronic muscle pain were treated with βCP-βCD (20mg/kg; p.o.) or vehicle (saline 0.9%, p.o.) and 90min. after, were perfused, the lumbar spinal cord collected, crioprotected, cut and submitted in an immunofluorescence protocol for Fos protein.The characterization tests indicated that βCP were efficiently incorporated into βCD. The oral treatment with βCP-βCD, at all doses tested, produced a significant (p0.05) reduction on mechanical hyperalgesia and a significant (p0.05) increase in muscle withdrawal thresholds, without produce any alteration in force. In addition, βCP-βCD was able to significantly (p0.05) decrease Fos expression in the superficial dorsal horn.Thus, βCP-βCD attenuates the non-inflammatory chronic muscle pain in mice and inhibits the Fos expression in the lumbar spinal cord. |
| Databáze: | OpenAIRE |
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