Heatstroke Effect on Brain Heme Oxygenase-1 in Rats
| Autor: | Ya Ting Wen, Tsung Ta Liu, Chun Chi Chen, Tien Jen Lin, Chi Chang Huang, Woon-Man Kung, Li Wei, Yuan Hung Wang, Yuh Feng Lin |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Central nervous system Poison control Brain damage Cell morphology Neuroprotection Gene Expression Regulation Enzymologic Rats Sprague-Dawley chemistry.chemical_compound heat stroke Internal medicine medicine Animals Heme Cerebral Cortex Short Research Communication business.industry Heatstroke heme oxygenase-1 Brain General Medicine medicine.disease Heme oxygenase cortical brain Endocrinology medicine.anatomical_structure chemistry Anesthesia Heme Oxygenase (Decyclizing) medicine.symptom business |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| Popis: | Exposure to high environmental temperature leading to increased core body temperature above 40°C and central nervous system abnormalities such as convulsions, delirium, or coma is defined as heat stroke. Studies in humans and animals indicate that the heat shock responses of the host contribute to multiple organ injury and death during heat stroke. Heme oxygenase-1 (HO-1)-a stress-responsive enzyme that catabolizes heme into iron, carbon monoxide, and biliverdin-has an important role in the neuroprotective mechanism against ischemic stroke. Here, we investigated the role of endogenous HO-1 in heat-induced brain damage in rats. RT-PCR results revealed that levels of HO-1 mRNA peaked at 0 h after heat exposure and immunoblot analysis revealed that the maximal protein expression occurred at 1 h post-heat exposure. Subsequently, we detected the HO-1 expression in the cortical brain cells and revealed the neuronal cell morphology. In conclusion, HO-1 is a potent protective molecule against heat-induced brain damage. Manipulation of HO-1 may provide a potential therapeutic approach for heat-related diseases. |
| Databáze: | OpenAIRE |
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