Expression of PTHrP and its cognate receptor in the rheumatoid synovial microcirculation
| Autor: | W Carley, Katherine J. Downey, D. E. Yocum, Janet L. Funk, Hongbing Wei, J. B. Benjamin |
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| Rok vydání: | 2002 |
| Předmět: |
musculoskeletal diseases
Cytoplasm medicine.medical_specialty Endothelium Peptide Hormones Biophysics Vasodilation Biochemistry Microcirculation Arthritis Rheumatoid Paracrine signalling Internal medicine In Situ Nick-End Labeling medicine Humans skin and connective tissue diseases Receptor Molecular Biology Cells Cultured Receptor Parathyroid Hormone Type 1 Cell Nucleus Chemistry Parathyroid hormone receptor Synovial Membrane Parathyroid Hormone-Related Protein Cell Biology musculoskeletal system Immunohistochemistry In vitro Cell biology Endocrinology medicine.anatomical_structure Apoptosis Receptors Parathyroid Hormone Endothelium Vascular hormones hormone substitutes and hormone antagonists |
| Zdroj: | Biochemical and Biophysical Research Communications. 297:890-897 |
| ISSN: | 0006-291X |
| Popis: | Parathyroid hormone-related protein (PTHrP), a multifunctional peptide that acts as a vasodilator as well as possible regulator of vascular development, is produced in increased amounts in the rheumatoid synovium. To understand whether PTHrP can contribute to the development and function of the rheumatoid microcirculation, studies were undertaken to identify and compare vascular sites of expression of PTHrP and its cognate receptor in the rheumatoid synovium and/or in cultured rheumatoid synovial endothelial cells. Endothelial cells, including apoptotic cells, as determined by TUNEL staining, were the primary site of vascular PTHrP expression in the rheumatoid synovium, a result confirmed in vitro in rheumatoid synovial microvascular endothelial cells. In contrast, the PTH/PTHrP receptor was primarily located in pericytes and smooth muscle cells within the vasculature. These results are consistent with a possible paracrine pathway for PTHrP action in the synovial microcirculation, wherein PTHrP peptides secreted by the synovial endothelium could act on surrounding PTH1R-positive pericytes and smooth muscle cells. |
| Databáze: | OpenAIRE |
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