Quantitative imaging of white and gray matter remyelination in the cuprizone demyelination model using the macromolecular proton fraction
| Autor: | Mikhail V. Svetlik, M Khodanovich, V Glazacheva, Yana Tyumentseva, A. E. Akulov, Vasily L. Yarnykh, Tatyana V Anan'ina, E Pan, Anna O Pishchelko |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine клетки-предшественники олигодендроцитов Corpus callosum Mice Myelin 0302 clinical medicine Gray Matter иммуногистохимия lcsh:QH301-705.5 reproductive and urinary physiology biology medicine.diagnostic_test Chemistry General Medicine купризоновая модель Magnetic Resonance Imaging White Matter Oligodendroglia myelin medicine.anatomical_structure Mesothelin cuprizone model immunohistochemistry Immunohistochemistry demyelination магнитно-резонансная томография medicine.medical_specialty macromolecular proton fraction oligodendrocytes демиелинизация Article oligodendrocyte precursors Cuprizone 03 medical and health sciences In vivo Internal medicine medicine Animals Remyelination олигодендроциты Oligodendrocyte Precursor Cells макромолекулярная протонная фракция urogenital system ремиелинизация Myelin Basic Protein Magnetic resonance imaging Oligodendrocyte Myelin basic protein Disease Models Animal remyelination 030104 developmental biology Endocrinology lcsh:Biology (General) nervous system biology.protein миелин MPF 030217 neurology & neurosurgery Demyelinating Diseases |
| Zdroj: | Cells. 2019. Vol. 8, № 10. P. 1204 (1-18) Cells Volume 8 Issue 10 Cells, Vol 8, Iss 10, p 1204 (2019) |
| Popis: | Macromolecular proton fraction (MPF) has been established as a quantitative clinically-targeted MRI myelin biomarker based on recent demyelination studies. This study aimed to assess the capability of MPF to quantify remyelination using the murine cuprizone-induced reversible demyelination model. MPF was measured in vivo using the fast single-point method in three animal groups (control, cuprizone-induced demyelination, and remyelination after cuprizone withdrawal) and compared to quantitative immunohistochemistry for myelin basic protein (MBP), myelinating oligodendrocytes (CNP-positive cells), and oligodendrocyte precursor cells (OPC, NG2-positive cells) in the corpus callosum, caudate putamen, hippocampus, and cortex. In the demyelination group, MPF, MBP-stained area, and oligodendrocyte count were significantly reduced, while OPC count was significantly increased as compared to both control and remyelination groups in all anatomic structures (p < 0.05). All variables were similar in the control and remyelination groups. MPF and MBP-stained area strongly correlated in each anatomic structure (Pearson&rsquo s correlation coefficients, r = 0.80&ndash 0.90, p < 0.001). MPF and MBP correlated positively with oligodendrocyte count (r = 0.70&ndash 0.84, p < 0.01 for MPF r = 0.81&ndash 0.92, p < 0.001 for MBP) and negatively with OPC count (r = &minus 0.69&ndash &minus 0.77, p < r = &minus 0.72&ndash 0.89, p < 0.01 for MBP). This study provides immunohistological validation of fast MPF mapping as a non-invasive tool for quantitative assessment of de- and remyelination in white and gray matter and indicates the feasibility of using MPF as a surrogate marker of reparative processes in demyelinating diseases. |
| Databáze: | OpenAIRE |
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