| Autor: |
Hernán Coaguila‐Llerena, Ronald Ordinola‐Zapata, Christopher Staley, Matthew Dietz, Ruoqiong Chen, Gisele Faria |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
International endodontic journal. 55(11) |
| ISSN: |
1365-2591 |
| Popis: |
The aim of the study was to assess biofilm removal efficacy of GentleWave System and passive ultrasonic irrigation (PUI).Twenty-two human mandibular molars with Vertucci's type II configuration in the mesial root were selected. Teeth were autoclaved, inoculated with dental plaque and incubated in a CDC biofilm reactor for two weeks. The mesial roots were instrumented up to 20.06 file (V-Taper) for the GentleWave group and up to 35.04 file (Vortex Blue) for PUI group. Irrigation was performed using GentleWave and PUI irrigation protocols (n = 11). Dentine debris on paper points samples were obtained for quantitative real-time polymerase chain reaction (qPCR) and 16S ribosomal RNA gene sequencing (next-generation aequencing-NGS). For qPCR, a non-parametric test (α = 0.05) was used. Next-generation sequencing data were analysed using mothur, with alpha diversity calculated as the Shannon and Chao1 indices and Bray-Curtis dissimilarities were used for beta diversity. Differences in alpha diversity and abundances of genera were evaluated using Kruskal-Wallis test. Differences in community composition were evaluated using analysis of similarity with Bonferroni correction for multiple comparisons.Quantitative real-time polymerase chain reaction results showed that the reduction estimated in percentages for both groups was equivalent (p .05). NGS analysis showed that both techniques promoted a significant reduction in reads and OTUs number (p .05). Shannon alpha diversity and Chao1 index showed no differences between pre- or post-treatment samples for both groups (p .05). Additionally, pre-treatment communities differed from post-treatment samples in both groups regarding bacterial taxa reduction (ANOSIM R = 0.50 and 0.55, p .001).Bacterial reduction in mesial roots of mandibular molars prepared to 35.04 with PUI was similar to those prepared to 20.06 with a multisonic irrigant activation system. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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