Comparison of the SARS-CoV-2 Rapid antigen test to the real star Sars-CoV-2 RT PCR kit
| Autor: | Michael Dreher, Alexander Krüttgen, Christian Cornelissen, Michael Kleines, Matthias Imöhl, Mathias W. Hornef |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Coronavirus disease 2019 (COVID-19) Short Communication viruses Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) 030106 microbiology Pcr assay Lateral flow assay Biology Sensitivity and Specificity 03 medical and health sciences COVID-19 Testing Antigen Virology Antigen assays Humans skin and connective tissue diseases Antigens Viral Immunoassay Cycle threshold Reverse Transcriptase Polymerase Chain Reaction SARS-CoV-2 fungi COVID-19 respiratory tract diseases body regions 030104 developmental biology Real-time polymerase chain reaction Rapid antigen test RNA Viral Reagent Kits Diagnostic |
| Zdroj: | Journal of Virological Methods |
| ISSN: | 0166-0934 |
| Popis: | Highlights • The sensitivity and specificity of the new Roche SARS-CoV-2 Rapid Antigen Test was evaluated. • We found a specificity of 96 %. • The sensitivity with samples with a Ct of < 25 was 100 % and gradually decreases to 22.2 % with cycle thresholds > = 35. • The new test might be useful to rapidly identify contagious individuals. There is an ongoing need for reliable antigen assays for timely and easy detection of individuals with acute SARS-CoV-2 infection. Using 75 swabs from patients previously tested positive by SARS-CoV-2 PCR and 75 swabs from patients previously tested negative by SARS-CoV-2 PCR, we investigated the sensitivity and specificity of the SARS-CoV-2 Rapid Antigen Test (Roche). We determined a specificity of 96 %. The assay’s sensitivity with samples with a cycle threshold of < 25, 25 - = 35 was 100 %, 95 %, 44.8 % and 22.2 %, respectively. We conclude that sensitivity and specificity of the antigen assay is inferior to the PCR assay. However, the antigen assay may be a quick and easy to perform alternative for differentiation of individuals contagious for SARS-CoV-2 from non-contagious individuals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect