Nephronectin promotes osteoblast differentiation via the epidermal growth factor-like repeats
| Autor: | Arun Seth, Shireen Kahai, Burton B. Yang, Shao-Chen Lee |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
MAPK/ERK pathway
Repetitive Sequences Amino Acid Cellular differentiation Biophysics Nephronectin Biology Matrix (biology) Biochemistry Cell Line Extracellular matrix 03 medical and health sciences Mice 0302 clinical medicine Structural Biology Epidermal growth factor Osteogenesis Gene expression Genetics medicine Animals Phosphorylation Extracellular Signal-Regulated MAP Kinases Molecular Biology 030304 developmental biology 0303 health sciences Extracellular Matrix Proteins Osteoblasts Matrix Epidermal Growth Factor Osteoblast Cell Differentiation Cell Biology Molecular biology ERK medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis |
| Zdroj: | FEBS letters. 584(1) |
| ISSN: | 1873-3468 |
| Popis: | We found that nephronectin was significantly down-regulated by TGF-β1. To determine the function of nephronectin in osteogenesis, we generated various constructs to produce stable MC3T3-E1 cell lines, expressing and secreting nephronectin protein, including full-length (Npnt), lacking EGF-like repeats (Np-MAM), and lacking RGD and MAM domains (Np-EGF). We demonstrated that nephronectin promotes differentiation during osteoblast differentiation and the EGF-like repeats were essential. Lack of these repeats resulted in inhibiting the change in morphology. Over-expression of nephronectin results in earlier formation of bone nodules than the vector control. ERK activation is essential for nephronectin-induced osteoblast differentiation. |
| Databáze: | OpenAIRE |
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