Gynostemma Pentaphyllum Extract Ameliorates High-Fat Diet-Induced Obesity in C57BL/6N Mice by Upregulating SIRT1
| Autor: | Joo Myung Moon, Su-Min Lim, Kyu Min Cha, Yoonhee Kim, So Mi Kim, Jae In Jung, Hyun Sook Lee, Tae Young Kim, Tae Kyu Oh, Eun Ji Kim, Jae Kyoung Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty obesity sirt1 gypenoside ginsenoside lcsh:TX341-641 White adipose tissue Article ampk 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Enhancer binding medicine Gynostemma pentaphyllum Carnitine adipocyte protein 2 Nutrition and Dietetics biology Triglyceride nutritional and metabolic diseases food and beverages biology.organism_classification gynostemma pentaphyllum Fatty acid synthase 030104 developmental biology Endocrinology chemistry 030220 oncology & carcinogenesis biology.protein lipids (amino acids peptides and proteins) Adipocyte hypertrophy lcsh:Nutrition. Foods and food supply Food Science medicine.drug |
| Zdroj: | Nutrients, Vol 11, Iss 10, p 2475 (2019) Nutrients Volume 11 Issue 10 |
| ISSN: | 2072-6643 |
| Popis: | Gynostemma pentaphyllum is widely used in Asia as a herbal medicine to treat type 2 diabetes, dyslipidemia, and inflammation. Here, we investigated the anti-obesity effect and underlying mechanism of G. pentaphyllum extract (GPE) enriched in gypenoside L, gypenoside LI, and ginsenoside Rg3 and obtained using a novel extraction method. Five-week-old male C57BL/6N mice were fed a control diet (CD), high-fat diet (HFD), HFD + 100 mg/kg body weight (BW)/day GPE (GPE 100), HFD + 300 mg/kg BW/day GPE (GPE 300), or HFD + 30 mg/kg BW/day Orlistat (Orlistat 30) for 8 weeks. The HFD-fed mice showed significant increases in body weight, fat mass, white adipose tissue, and adipocyte hypertrophy compared to the CD group but GPE inhibited those increases. GPE reduced serum levels of triglyceride, total cholesterol, and LDL-cholesterol, without affecting HDL-cholesterol. GPE significantly increased AMPK activation and suppressed adipogenesis by decreasing the mRNA expression of CCAAT/enhancer binding protein-&alpha (C/EBP&alpha ), peroxisome proliferator-activated receptor-&gamma (PPAR&gamma ), sterol regulatory element-binding protein-1c (SREBP1c), PPAR&gamma coactivator-1&alpha fatty acid synthase (FAS), adipocyte protein 2 (AP2), and sirtuin 1 (SIRT1) and by increasing that of carnitine palmitoyltransferase (CPT1) and hormone- sensitive lipase (HSL). This study demonstrated the ameliorative effect of GPE on obesity and elucidated the underlying molecular mechanism. |
| Databáze: | OpenAIRE |
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