Expression of CD27 on Murine Hematopoietic Stem and Progenitor Cells
| Autor: | A. Elena Searles, Mariluz P. Mojica, Gerald J. Spangrude, L. Jeanne Pierce, Robert L. Phillips, Ihor R. Lemischka, Anne Wiesmann |
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| Rok vydání: | 2000 |
| Předmět: |
Immunology
Population Biology Mice 03 medical and health sciences 0302 clinical medicine Animals Immunology and Allergy Progenitor cell education 030304 developmental biology Interleukin 3 0303 health sciences education.field_of_study hemic and immune systems Hematopoietic Stem Cells Molecular biology In vitro Tumor Necrosis Factor Receptor Superfamily Member 7 Mice Inbred C57BL Endothelial stem cell Transplantation Haematopoiesis Infectious Diseases Stem cell 030215 immunology |
| Zdroj: | Immunity. 12:193-199 |
| ISSN: | 1074-7613 |
| DOI: | 10.1016/s1074-7613(00)80172-7 |
| Popis: | Hematopoietic stem cells (HSC) are defined by self-renewal and multilineage differentiation potentials. In order to uncover the genetic program of HSC, we utilized high-density arrays to compare gene expression in highly purified mouse HSC and their mature progeny. One molecule specifically expressed in immature cells is CD27, a member of the TNF receptor family previously shown to play roles in lymphoid proliferation, differentiation, and apoptosis. We show here that the CD27 protein is expressed by about 90% of cells in a purified HSC population. Interestingly, the CD27pos cells are enriched for cells with short-term hematopoietic activities (colony forming potential in vivo and in vitro), while the minority CD27neg population is more effective in clonal long-term transplantation. |
| Databáze: | OpenAIRE |
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