Dendritic cells derived from bone marrow and CD34+ selected blood progenitor cells of myeloma patients, cultured in serum-free media, do not contain the Kaposi sarcoma herpesvirus genome
| Autor: | W Mair, T F Schultz, Julie Sheldon, Manfred Mitterer, M Vachula, D Gatti, P Coser |
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| Rok vydání: | 1998 |
| Předmět: |
biology
Stem Cells CD34 Antigens CD34 Dendritic Cells Genome Viral Hematology Dendritic cell biology.organism_classification medicine.disease Virology Culture Media Serum-Free medicine.anatomical_structure Herpesvirus 8 Human Cancer research medicine Humans Gammaherpesvirinae Bone marrow Stem cell Progenitor cell Multiple Myeloma Antigen-presenting cell Cells Cultured Multiple myeloma |
| Zdroj: | British Journal of Haematology. 102:1338-1340 |
| ISSN: | 0007-1048 |
| DOI: | 10.1046/j.1365-2141.1998.00894.x |
| Popis: | The aim of our study was to test if dendritic cells contain the KSHV genome. CD34+ peripheral blood progenitor cells (PBPC) and bone marrow mononuclear cells were cultured in X-VIVO 15 medium supplemented with GM-CSF and TNF-alpha in gas-permeable containers. Dendritic cells were identified morphologically and immunophenotypically. The KSHV genome was not identified in any of the cases using a nested primer PCR approach. Serological analysis corroborated the molecular findings: no antibodies for KSHV were found in any of the multiple myeloma patients. These data are of importance when considering use of DC for therapeutic approaches in multiple myeloma. |
| Databáze: | OpenAIRE |
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