Cadherin-13 in primary and blast crisis chronic myeloid leukaemia: declining expression and negative correlation with theBCR/ABLfusion gene
| Autor: | Mu Hj, Yunfeng Shen, Zeng Yj, Xie R, Yuanqiang Jiang |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Microbiology (medical) Clinical Biochemistry Immunology Fusion Proteins bcr-abl Biology Microbiology Fusion gene Myelogenous Leukemia Myelogenous Chronic BCR-ABL Positive hemic and lymphatic diseases medicine Humans Immunology and Allergy Genes Tumor Suppressor neoplasms Gene Electrophoresis Agar Gel ABL Gene Expression Regulation Leukemic Reverse Transcriptase Polymerase Chain Reaction Biochemistry (medical) breakpoint cluster region Cadherins medicine.disease Reverse transcriptase Leukemia Infectious Diseases Disease Progression Cancer research Blast Crisis K562 cells |
| Zdroj: | British Journal of Biomedical Science. 66:20-24 |
| ISSN: | 0967-4845 |
| Popis: | Expression of the CDH13 gene in chronic myeloid leukaemia (CML) patients at different clinical stages, and its relationship with the BCR/ABL fusion gene, is investigated. Expression of the CDH13 gene and the BCR/ABL fuse gene is investigated in peripheral blood from 30 healthy adults, 25 primary CML patients and 25 CML patients in blast crisis using the EvaGreen real-time reverse transcriptase polymerase chain reaction (RT-PCR). Results showed that BCR/ABL fusion gene expression in the blast crisis CML patients was 4.72-fold higher than that in patients with primary CML. Expression of CDH13 mRNA in primary and blast crisis CML patients was lower than in the healthy adults, reduced 64% and 75%, respectively. Expression of CDH13 showed a negative correlation with the BCR/ABL fusion gene. The data indicate that the decline of CDH13 expression accompanied the different clinical stages of CML and probably was involved in over-expression of the BCR/ABL fusion gene. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|