Endothelin-1 induced desensitization in primary afferent neurons
| Autor: | Sherika N. Smith, Sarah M. Sweitzer, Terika P. Smith |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Primary Cell Culture Pharmacology Article Rats Sprague-Dawley chemistry.chemical_compound Calcium imaging Dorsal root ganglion Desensitization (telecommunications) Ganglia Spinal medicine Animals Neurons Afferent Sensitization Endothelin-1 business.industry General Neuroscience medicine.anatomical_structure Nociception chemistry Capsaicin Anesthesia Sensory System Agents Hyperalgesia Calcium medicine.symptom business Priming (psychology) |
| Zdroj: | Neuroscience Letters. 582:59-64 |
| ISSN: | 0304-3940 |
| Popis: | Endothelin-1 (ET-1) is a known algogen that causes acute pain and sensitization in humans and spontaneous nociceptive behaviors when injected into the periphery in rats, and is elevated during vaso-occlusive episodes (VOEs) in sickle cell disease (SCD) patients. Previously, our lab has shown that a priming dose of ET-1 produces sensitization to capsaicin-induce secondary hyperalgesia. The goal of this study was to determine if the sensitization induced by ET-1 priming is occurring at the level of the primary afferent neuron. Calcium imaging in cultured dorsal root ganglion (DRG) neurons was utilized to examine the effects of ET-1 on primary afferent neurons. ET-1 induces [Ca2+]i transients in unprimed cells. ET-1 induced [Ca2+]i transients are attenuated by priming with ET-1. This priming effect occurs whether the priming dose is given 0–4 days prior to the challenge dose. Similarly, ET-1 priming decreases capsaicin-induced [Ca2+]i transients. At the level of the primary afferent neuron, ET-1 priming has a desensitizing effect on challenge exposures to ET-1 and capsaicin. |
| Databáze: | OpenAIRE |
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