Differential inhibitory and activating NK cell receptor levels and NK/NKT-like cell functionality in chronic and recovered stages of chikungunya
| Autor: | Anuradha S. Tripathy, Mohini A. Ganu, Subrat Thanapati |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male RNA viruses Viral Diseases Physiology Cell lcsh:Medicine NK cells Pathology and Laboratory Medicine 0302 clinical medicine Spectrum Analysis Techniques Immune Physiology Cellular types Cytotoxic T cell Enzyme-Linked Immunoassays Receptor Child lcsh:Science Innate Immune System Multidisciplinary Chikungunya Virus biology medicine.diagnostic_test Immune cells hemic and immune systems Middle Aged Flow Cytometry Killer Cells Natural medicine.anatomical_structure Infectious Diseases Medical Microbiology Spectrophotometry Viral Pathogens Viruses Receptors Natural Killer Cell White blood cells Cytokines Female Cytophotometry Pathogens Research Article Neglected Tropical Diseases Adult Cell biology Blood cells Adolescent Patients CD3 Alphaviruses 030231 tropical medicine Immunology chemical and pharmacologic phenomena Research and Analysis Methods Microbiology Virus Flow cytometry Togaviruses 03 medical and health sciences Interferon-gamma Young Adult medicine Humans Immunoassays Molecular Biology Techniques Microbial Pathogens Molecular Biology Aged Medicine and health sciences Biology and life sciences Tumor Necrosis Factor-alpha lcsh:R Organisms Chikungunya Infection Molecular Development NKG2D Tropical Diseases Health Care 030104 developmental biology Perforin Animal cells Immune System biology.protein Immunologic Techniques Chikungunya Fever Natural Killer T-Cells lcsh:Q Developmental Biology Cloning |
| Zdroj: | PLoS ONE, Vol 12, Iss 11, p e0188342 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The role of natural killer (NK; CD3-CD56+)/NKT (CD3+CD56+)-like cells in chikungunya virus (CHIKV) disease progression/recovery remains unclear. Here, we investigated the expression profiles and function of NK and NKT-like cells from 35 chronic chikungunya patients, 30 recovered individuals, and 69 controls. Percentage of NKT-like cells was low in chronic chikungunya patients. NKp30+, CD244+, DNAM-1+, and NKG2D+ NK cell percentages were also lower (MFI and/or percentage), while those of CD94+ and NKG2A+ NKT-like cells were higher (MFI and/or percentage) in chronic patients than in recovered subjects. IFN-γ and TNF-α expression on NKT-like cells was high in the chronic patients, while only IFN-γ expression on NK cells was high in the recovered individuals. Furthermore, percentage of perforin+NK cells was low in the chronic patients. Lower cytotoxic activity was observed in the chronic patients than in the controls. CD107a expression on NK and NKT-like cells post anti-CD94/anti-NKG2A blocking was comparable among the patients and controls. Upregulated inhibitory and downregulated activating NK receptor expressions on NK/NKT-like cells, lower perforin+ and CD107a+NK cells are likely responsible for inhibiting the NK and NKT-like cell function in the chronic stage of chikungunya. Therefore, deregulation of NKR expression might underlie CHIKV-induced chronicity. |
| Databáze: | OpenAIRE |
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