Trans-neuronal transduction of spinal neurons following cortical injection and anterograde axonal transport of a bicistronic AAV1 vector
| Autor: | Thomas H. Hutson, Claudia Kathe, Lawrence D. F. Moon |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
viruses Central nervous system Gene delivery Biology Bioinformatics Axonal Transport Article 03 medical and health sciences Transduction (genetics) Transduction Genetic Genetics medicine Animals Molecular Biology Neurons Reporter gene Gene Transfer Techniques Dependovirus Spinal cord Anterograde axonal transport Rats 030104 developmental biology medicine.anatomical_structure Spinal Cord nervous system Corticospinal tract Axoplasmic transport Molecular Medicine Female Sensorimotor Cortex Neuroscience |
| Zdroj: | Gene Therapy. 23:231-236 |
| ISSN: | 1476-5462 0969-7128 |
| Popis: | Adeno-associated viral (AAV) vectors are one of the most promising gene delivery systems to the central nervous system. We now report, that AAV1 can be used to express transgenes trans-neuronally in neurons distant from the injection site. Specifically, intracortical injection of a bicistronic AAV1 vector trans-neuronally transduced spinal neurons as shown by fluorescence microscopy, the presence of AAV genome and AAV transcript in the contralateral spinal cord. Prior pyramidotomy abolished spinal transduction, confirming anterograde axonal transport of AAV1 in the corticospinal tract. These observations demonstrate the potential of bicistronic AAV1 for trans-neuronal expression of therapeutic transgenes in neurological disorders or reporter genes in connectivity studies. |
| Databáze: | OpenAIRE |
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