The neonatal marmoset monkey ovary is very primitive exhibiting many oogonia
| Autor: | Bentolhoda Fereydouni, Nelia Aeckerle, Ruediger Behr, Stefan Schlatt, Charis Drummer |
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| Rok vydání: | 2014 |
| Předmět: |
Pluripotent Stem Cells
endocrine system Embryology Cellular differentiation Ovary Real-Time Polymerase Chain Reaction Immunoenzyme Techniques Andrology Mice Oogonia Endocrinology biology.animal medicine Animals Humans Primate RNA Messenger Cells Cultured Genetics biology Reverse Transcriptase Polymerase Chain Reaction Research RNA-Binding Proteins Obstetrics and Gynecology Marmoset Callithrix Cell Differentiation Cell Biology biology.organism_classification Meiosis Germ Cells medicine.anatomical_structure Animals Newborn Reproductive Medicine Octamer Transcription Factors Female Stem cell Development of the gonads Biomarkers Germ cell |
| Zdroj: | Reproduction (Cambridge, England) |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep-14-0068 |
| Popis: | Oogonia are characterized by diploidy and mitotic proliferation. Human and mouse oogonia express several factors such as OCT4, which are characteristic of pluripotent cells. In human, almost all oogonia enter meiosis between weeks 9 and 22 of prenatal development or undergo mitotic arrest and subsequent elimination from the ovary. As a consequence, neonatal human ovaries generally lack oogonia. The same was found in neonatal ovaries of the rhesus monkey, a representative of the old world monkeys (Catarrhini). By contrast, proliferating oogonia were found in adult prosimians (now called Strepsirrhini), which is a group of ‘lower’ primates. The common marmoset monkey (Callithrix jacchus) belongs to the new world monkeys (Platyrrhini) and is increasingly used in reproductive biology and stem cell research. However, ovarian development in the marmoset monkey has not been widely investigated. Herein, we show that the neonatal marmoset ovary has an extremely immature histological appearance compared with the human ovary. It contains numerous oogonia expressing the pluripotency factors OCT4A, SALL4, and LIN28A (LIN28). The pluripotency factor-positive germ cells also express the proliferation marker MKI67 (Ki-67), which has previously been shown in the human ovary to be restricted to premeiotic germ cells. Together, the data demonstrate the primitiveness of the neonatal marmoset ovary compared with human. This study may introduce the marmoset monkey as a non-human primate model to experimentally study the aspects of primate primitive gonad development, follicle assembly, and germ cell biologyin vivo. |
| Databáze: | OpenAIRE |
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